Huanglongbing (HLB) is a citrus disease that affects the growth of fibrous roots trees. This means HLB-affected trees may have reduced root volume and impact water uptake. A greenhouse study was conducted from October 2019 to July 2021 at Citrus Research Education Center (CREC) in Lake Alfred, FL, evaluate development under deficit irrigation system. The objective assess on tree growth, availab...

A new huanglongbing (HLB) "Candidatus Liberibacter" species is genetically characterized, and the bacterium is designated "Candidatus Liberibacter psyllaurous." This bacterium infects the psyllid Bactericera cockerelli and its solanaceous host plants potato and tomato, potentially resulting in "psyllid yellowing." Host plant-dependent HLB transmission and variation in psyllid infection frequenc...

Root samples of 'Sanhu' red tangerine trees infected with and without Candidatus Liberibacter asiaticus (CLas) were collected at 50 days post inoculation and subjected to RNA-sequencing and isobaric tags for relative and absolute quantification (iTRAQ) to profile the differentially expressed genes (DEGs) and proteins (DEPs), respectively. Quantitative real-time PCR was subsequently used to conf...

Nuclear bodies (NBs) are structures that concentrate proteins, RNAs, and ribonucleoproteins that perform functions essential to gene expression. How NBs assemble is not well understood. We studied the Drosophila histone locus body (HLB), a NB that concentrates factors required for histone mRNA biosynthesis at the replication-dependent histone gene locus. We coupled biochemical analysis with con...

Citrus Huanglongbing (HLB), the most destructive citrus disease, can be transmitted by psyllids and diseased budwoods. Although the final symptoms of the two main HLB transmission ways were similar and hard to distinguish, the host responses might be different. In this study, the global gene changes in leaves of ponkan (Citrus reticulata) mandarin trees following psyllid-transmission of HLB wer...

Interstocks have been used in fruit tree cultivation to regulate size and improve production quality. In this study, several Huanglongbing (HLB)-tolerant open-pollinated pummelo interstock candidates were evaluated as interstocks between the Swingle rootstock ‘Valencia’ scion, with serving control interstock. After 5 years field, most trees did not exhibit visual HLB symptoms, although infected...

Commercial sweet orange cultivars lack resistance to Huanglongbing (HLB), a serious phloem limited bacterial disease that is usually fatal. In order to develop sustained disease resistance to HLB, transgenic sweet orange cultivars 'Hamlin' and 'Valencia' expressing an Arabidopsis thaliana NPR1 gene under the control of a constitutive CaMV 35S promoter or a phloem specific Arabidopsis SUC2 (AtSU...

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is one of the most economically destructive pests of citrus worldwide. D. citri vector the phloem-inhabiting bacterium Candidatus Liberibacter asiaticus, which is the presumed causal agent of huanglongbing (HLB) disease in citrus in North America. HLB, otherwise known as citrus greening disease, is the greatest biotic t...