We have analyzed the organization of the homogeneously staining regions (HSRs) in chromosomes from a methotrexate-resistant mouse melanoma cell line. Fluorescence in situ hybridization techniques were used to localize satellite DNA sequences and the amplified copies of the dihydrofolate reductase (DHFR) gene that confer drug-resistance, in combination with immunofluorescence using antibody prob...

This paper studies the Turing degrees of various properties defined for universal numberings, that is, for numberings which list all partial-recursive functions. In particular properties relating to the domain of the corresponding functions are investigated like the set DEQ of all pairs of indices of functions with the same domain, the set DMIN of all minimal indices of sets and DMIN∗ of all in...

Cytogenetic investigation of a nodal diffuse large B cell lymphoma carrying an IGH-BCL2-fusion revealed a homogeneously staining region at chromosome 1p21-22. Fluorescence in situ hybridisation (FISH) demonstrated heterogeneous BCL10 gene amplification in tumour cells. Immunohistochemistry showed heterogeneous over-expression of the protein in the nuclei of tumour cells, similar to that seen in...

The chromosomal G/R bands are alternating domains differing in their nucleotide sequence biases. The bands are also related to the time of replication: pulse-labeling during S phase makes the replication sites as visible as replication bands that are close to the G/R bands in mitotic chromosomes. We previously showed that a plasmid bearing a mammalian replication origin efficiently generated a ...

We have isolated cDNA clones complementary to a c-Ki-ras cellular oncogene that is amplified in Y1 mouse adrenal tumor cells, with the amplified sequences located on double-minute chromatin bodies (DMs) and homogeneously staining chromosomal regions (HSRs). Characterization of the cDNAs included the isolation of corresponding genomic clones, Northern blot analysis of RNA, and DNA sequence analy...

Objective A peripherally inserted central catheter (PICC) is a safe and effective drug delivery method of chemoradiotherapy for lung cancer. However, the risk vascular damage to patients who require PICC implantation during radiotherapy may be increased. This study compared dosimetry differences in blood vessel region, as well planning target volume other organs at between two volumetric modula...

Adaptive power allocation (PA) algorithms based on opti6 mization of the minimum distance dmin between signal points at the 7 receiver side are investigated in spatial modulation (SM) systems. First, a 8 closed-form solution of the optimal maximum-dmin-aided PA algorithm is 9 derived in the case of two transmit antennas (TAs). Moreover, for a higher 10 number of TAs, we propose a numerical appr...