objective(s): adeno-associated virus type 2 (aav2) vectors are widely used for both experimental and clinical gene therapy. a recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. in this study, a fast and simple method is presented to generate aav2 vector helper plasmids encodin...

The objective is to access the role of Roux-en-Y gastric bypass (RYGB) biliopancreatic limb (BPL) length in type 2 diabetes (T2D) outcomes.RYGB is more effective than medical intervention for T2D treatment in obese patients. Despite the scarcity of available data, previous reports suggest that modifications of the RYGB limb lengths could improve the antidiabetic effects of the surgery.A cohort ...

BACKGROUND The aim of the study was to explore Fc mutations of a humanised anti-Lewis-Y antibody (IgG1) hu3S193 as a strategy to improve therapeutic ratios for therapeutic payload delivery. METHODS Four hu3S193 variants (I253A, H310A, H435A and I253A/H310A) were generated via site-directed mutagenesis and radiolabelled with diagnostic isotopes iodine-125 or indium-111. Biodistribution studies...

This paper presents a method to improve the translation of polysemous nouns, leveraging on their previous occurrence as the head of a compound noun phrase. First, the occurrences are identified through pattern matching rules, which detect occurrences of an XY compound followed closely by a potentially coreferent occurrence of Y , such as “Mooncakes . . . cakes . . .”. Second, two strategies are...

The multi-path algorithm for finding hamilton cycles in a graph G is described in the book by Christofides. It is an intelligent exhaustive search for a hamilton cycle. In this paper we describe how the algorithm can be improved in two ways: (1) by detecting small separating sets M for which G–M has more than |M| components; and (2) by detecting bipartitions (X,Y), where |X|<|Y|.

The well-known theorem of Dvoretzky [1] states that convex bodies of high dimension have low dimensional sections which are almost spherical. More precisely, the theorem states that for every integer k and every e > 0 there is an integer n(k, e) such that any Banach space X with dimension > n(k, e) has a subspace Y of dimension k with d{Y,l\) < 1 + e. Here d(Y, l£) denotes the Banach Mazur dist...

A multiplex PCR assay capable of amplifying 20 Y-chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the ‘‘extended haplotype’’ used in Europe (DYS19, DYS385, DYS389I/II, DYS390, DYS391, DYS392, DYS393, and YCAII) plus additional polymorphic Y STRs (DYS437, DYS438, DYS439, DYS447, DYS448, DYS388, DYS426, GATA A7.1,...

This thesis investigates the use of anomaly dete tion te hniques for on-line intrusion dete tion. A detailed analysis of methods introdu ed by Forrest et al. is presented and an improved hara terization of intrusions is derived. A novel approa h to integrating this hara terization into an on-line intrusion dete tion system is developed and implemented. Finally, experiments examining the eÆ ien ...

In this paper, we investigate the question of triviality of the rational Chow groups of complete intersections in projective spaces and obtain improved bounds for this triviality to hold. Along the way, we study the dimension and nonemptiness of some Hilbert schemes of fat r-planes contained in a complete intersection Y , generalizing well-known results on the Fano varieties of r-planes contain...