AIMS To optimize peroxidase production by Streptomyces sp. strain BSII#1, up to 3 l culture volumes. METHODS AND RESULTS Peroxidase production by Streptomyces sp. strain BSII#1 was optimized in terms of production temperature and pH and the use of lignin-based model chemical inducers. The highest peroxidase activity (1·30 ± 0·04 U ml(-1) ) in 10 ml culture volume was achieved in a complex pro...

Conventional airlift contactor (UT-ALC) was modified to improve its performance. The modified system (CDT-ALC) was tested for gas hold-up characteristics. Hydrodynamic environment depends on gas hold-up. New system reported 50 % higher gas hold-up. Linear relation between superficial gas velocity and fractional gas hold-up were developed from experimental data for both the reactors.

Batch and continuous production of the extracellular heme glycoprotein chloroperoxidase (CPO) was studied with an airlift fermentor. We induced Caldariomyces fumago CMI 89362 to form pellets by transferring a small inoculum volume in preculture prior to growth in a 1-liter fermentor. Continuous replacement of the fructose-salts medium (dilution rate, 0.008 h) supported continuous CPO formation ...