The antitumor protein macromomycin is a single chain polypeptide of 112 amino acid residues cross-linked by two intramolecular disulfide bonds. The protein was reduced and S-alkylated with 2-mercaptoethanol in 8 M urea followed by treatment with iodoacetic acid. Tryptic digestion of tetra-S-carboxymethyl macromomycin gave four tryptic peptides which were fractionated by gel permeation on Sephad...

We have previously demonstrated coordinate inductions of c-fos, c-jun, jun B, and jun D in cardiac myocytes exposed to hypoxia for 2 to 4 hours. Induction of these transcripts occurred before any significant loss of intracellular ATP. In the present study, the origin of the signal(s) that regulates immediate-early gene induction was investigated by comparing the effects of hypoxia with those of...

The heat-shock (HS) response is a ubiquitous cellular response to stress, involving the transcriptional activation of HS genes. Reactive oxygen species (ROS) have been shown to regulate the activity of a number of transcription factors. We investigated the redox regulation of the stress response and report here that in the human pre-monocytic line U937 cells, H2O2 induced a concentration-depend...

Mutant A11, a mutant of Saccharomycopsis fibuligera Sdu with low acid and neutral trehalase was found to accumulate over 18% (w/w) trehalose from starch in its cells. In this study, trehalose-6-phosphate synthase (Tps1) was purified to homogeneity from this mutant, with a 30-fold increase in the specific enzyme activity, as compared to the concentrated cell-free extract, from initial cells. The...

Orostachys cartilaginous of Crassulaceae family is a plant native to the Changbai Mountain area, China. Although O. has various medicinal values, its product development and production are restricted by insufficient resource available. cell cultures possess an efficient antibacterial effect against Bacillus subtilis, but underlying mechanism not clear yet. Therefore, this study investigated eff...

In the search for tumor sensitivity test to chemotherapeutic agents succinic dehydrogenase activity by neotetrazolium chloride was measured colorimetrically. The rate of formazan production was proportional to the amount of Ehrlich ascites tumor cells, accelerated by the addition of ascorbic acid, and inhibited by the addition of iodoacetic acid. The inhibitory rate of formazan production of tu...