Isolation of plasmid DNA from viridans group streptococci is difficult, and preparations are often heavily contaminated with chromosomal DNA. We developed a simple protocol to isolate pure plasmid DNA for use in different molecular techniques, including automated sequencing. The protocol is also applicable for plasmid isolation from Staphylococcus aureus. In addition, the protocol allows isolat...

Student, Department of Computer Science Engineering (Data Science), Ajay Kumar Garg College,Ghaziabad

A facultative fungal parasite, Arkansas Fungus 18 (ARF18), was isolated from at least 10 different sources of Heterodera glycines in different areas in the United States. Techniques used for isolation and in vitro evaluation of fungal parasites of H. glycines were described. Successful isolation of probable egg parasites depends on isolation from individual eggs. Selective isolation and a simpl...

background and objectives: many members of the amaryllidaceae are regarded as toxic. the toxic constituents that occur in the whole family are referred to as the amaryllidaceae alkaloids. the main aim of this study was the identification of alkaloid compounds from galanthus transcaucasicus fomin, a medicinal plant from amaryllidaceae. methods: planar and column chromatography techniques were us...

lipases catalyze hydrolysis and synthesis of triacylglycerols. these enzymes,especially the microbial ones, have wide commercial and industrial usage. bacillus subtilis lipase has interesting properties such as small size and tolerance to basic ph; therefore, developing techniques for its recognition and isolation is the focus of research in many laboratories. in the present study, two factors ...