Frontal fibrosing alopecia (FFA) is the most common cicatricial alopecia, whose prevalence has greatly increased, mainly in post-menopausal women, results from irreversible epithelial hair follicle (HF) stem cell (eHFSC) damage. Exposure to ingredients commonly found personal care products might trigger FFA-related immune responses. In fact, 76.2% of patients with FFA and/or LPP show type IV se...

The mammalian E3 ubiquitin ligases RNF8 and RNF168 facilitate recruitment of the DNA damage response protein 53BP1 to sites of DNA double-strand breaks (DSBs). The mechanism involves recruitment of RNF8, followed by recruitment of RNF168, which ubiquitinates histones H2A/H2AX on K15. 53BP1 then binds to nucleosomes at sites of DNA DSBs by recognizing, in addition to methyl marks, histone H2A/H2...

PURPOSE To demonstrate the effects of hypoxia on proliferation and differentiation of human limbal epithelial cells in vitro. METHODS Primary human limbal epithelial cells were harvested from the rim of donor corneas. Colony-forming efficiency (CFE) and cell proliferation were observed in standard (20% O(2)) or hypoxic (2% O(2)) culture conditions. Cell cycle, forward scatter (FSC) and side s...

BACKGROUND Kaposi sarcoma (KS) occurs mainly in immunocompromised patients and is strongly associated with infection with human herpesvirus 8 (HHV-8; also known as "KS-associated herpesvirus"). We hypothesized that KS is linked to deficiencies in specific anti-HHV-8 T cell immunity. METHODS We studied asymptomatic HHV-8 carriers coinfected with human immunodeficiency virus (HIV; n = 23) and p...

A method to determine the surface azimuthal anchoring energy of a nematic liquid crystal is proposed. The technique implies the measurement of the director deviation on the cell substrate as a function of strength and direction of the applied magnetic field. As an example, the dependence of the azimuthal anchoring coefficient on the exposure time is measured at the interface between the nematic...

Chemotherapy-induced alopecia (CIA) is a highly distressing side effect of cancer therapy, and taxanes like paclitaxel (PTX) cause severe often permanent CIA. Yet, strategies that selectively protect healthy cells, but not remain elusive. Here, we turned to ALRN-6924, new clinical-stage MDM2/MDMX dual inhibitor activates p53 upregulate p21 thus transiently arrests cell cycle in p53-wild-type p5...

Chemotherapy-induced alopecia (CIA) is a deeply distressing adverse effect of cancer therapy, and novel CIA-protective strategies are urgently needed. Previously, we had shown that ALRN-6924, new clinical-stage MDM2/MDMX inhibitor selectively activates p53 to upregulate p21 thus transiently arrests the cell cycle in healthy cells only, protects hair follicles (HFs) from taxane-induced damage ex...