The demonstration that oxidation-reduction reactions mediated by the pyridine nucleotide coenzymes occur with the transfer of hydrogen between substrate and coenzyme (1) has led to the use of hydrogen isotopes to measure the occurrence of the specific coupling of reactions in the intact cell and organism (2-4). Several problems associated with the cellular organization and integration of the fe...

Translational readthrough gives rise to low abundance proteins with C-terminal extensions beyond the stop codon. To identify functional translational readthrough, we estimated the readthrough propensity (RTP) of all stop codon contexts of the human genome by a new regression model in silico, identified a nucleotide consensus motif for high RTP by using this model, and analyzed all readthrough e...

 The purpose of this study was to investigate the therapeutic effects of natural products like propolis on biochemical and hematologic parameters in carp (Cyprinus carpio, Linnaeus 1758) exposed to arsenic. In this study fish were exposed to 0.01 mg/L arsenic and 10 mg/L propolis for seven days. Our results indicated that triglyceride, urea, total cholesterol, cobalt, ALT (alanine amino transfe...

introduction: the use of antioxidants before high-intensity training, which leads to the release of free radicals and muscle injuries, can result in reduced damage during exercise. accordingly, in this study, we aimed to evaluate the effect of selenium supplement intake on oxidative stress, following exhaustive aerobic exercise among young physically-active females. methods: in this quasi-exper...

objective(s): carbon nanotubes have unique mechanical, electrical, and thermal properties, with potential different applications in nanomedicine, electronics, and other industries. these new applications of carbon nanotubes in different industries lead to the increased exposure risk of nanomaterials to human. up to now, all aspects of carbon nanotubes toxicity are not completely clear following...

A new NAD -isomer was prepared, in which the D-ribose of the adenosine moiety was substituted by the enantiomeric L-ribose. As compared to nicotinamide-adenine-dinucleotide (NAD) and NADH the coenzyme isomer (D,L)-NAD and its dihydroform (D,L)-NADH are far less tightly bound to lactate dehydrogenase and alcohol dehydrogenase from horse liver. In the presence of the second substrate (D,L)-NAD an...

The DNA sequence of the gene encoding the early and specific immunogenic protein P36 of Mycoplasma hyopneumoniae has been determined. Comparison of the DNA sequence and the deduced amino acid sequence of P36 with known genes and proteins in data banks indicated that P36 is a L-lactate dehydrogenase (LDH) (EC 1.1.1.27). Biochemical analysis of protein P36 expressed from the cloned gene in Escher...

The activities of pyridine nucleotide-independent d- and l-lactate dehydrogenases were detected in membranes from Rhodospirillum rubrum grown under aerobic and phototrophic conditions. Crossed immunoelectrophoretic analysis revealed two antigenically distinct enzymes that were further distinguished by specificity for d- and l-stereoisomers of lactate and by the sensitivity of the d-lactate dehy...

Lactate dehydrogenase and malate are the two main alpha-hydroxy acid dehydrogenases in human body. We investigated catalytic properties of lactate LDHC, LDHL6A MDH1 on aromatic α-keto acids phenylpyruvic acid, p-hydroxyphenylpyruvic 3,4-dihydroxyphenylpyruvic acid. The optimum temperatures for LDHL6A, 37 °C, 35 45 respectively; pH is 6.5, 5.5, respectively. Km LDHC were 0.90 mM 0.92 mM, has a h...

The patient is a 7-year-old girl with a history of repaired gastroschisis and short gut syndrome. She presented with a 4-day history of progressive encephalopathy, truncal ataxia, and omnidirectional gaze-evoked nystagmus. Peripheral lactate was 7.1 mmol/L. Serum thiamine level was decreased (,5 nmol/L). Brain magnetic resonance with spectroscopy is shown in the figure. A clinical diagnosis of ...