BACKGROUND Rapid detection of biological threat agents is critical for timely therapeutic administration. Fluorogenic PCR provides a rapid, sensitive, and specific tool for molecular identification of these agents. We compared the performance of assays for 7 biological threat agents on the Idaho Technology, Inc. R.A.P.I.D., the Roche LightCycler, and the Cepheid Smart Cycler. METHODS Real-tim...

cholera is an infection of the small intestines caused by the bacterium v. cholerae. it is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. the major virulence factor produced by v. cholerae during infection is the cholera toxin. total mrna extraction and reverse transcription was performed for making ctxab cdna. relative real-time...

Background and objective: The assessment of human epidermal growth factor receptor 2 (HER2) status has become of great importance in the diagnosis of breast cancer. The aim of this study was to investigate the diagnostic value of quantitative Polymerase Chain Reaction (qPCR) and Chromogenic In Situ Hybridization (CISH) to assess HER2 status of biopsy specimens. <...

background pertussis is a respiratory and contagious disease which is mostly caused by bordetella pertussis and b. parapertussis. it usually spreads from person to personduring the incubation or catarrhal phase of the disease. despite of large-scale vaccination, whooping cough is still an endemic disease with several outbreaks. objectives the aim of this study was to determine the prevalence of...

In the last decade, the use of culture-independent methods for detecting indicator organisms and pathogens in recreational waters has increased and has led to heightened interest in their use for routine water quality monitoring. However, a thorough understanding of the persistence of genetic markers in environmental waters is lacking. In the present study, we evaluate the persistence of entero...

Abstract Background Circulating tumor cells represent an opportunity for the assessment of early recurrent disease or real-time tracing cancer. Glucose Regulated Protein 78 ( GRP78 ) is known in literature as a stress factor endometrial We aimed to investigate importance gene by targeting traces circulating cell fluids patients with Type 1 cancer, examining cell-free RNAs patients’ samples and ...

As the optimization of electrochemical performance lithium-ion batteries by adjustment composition cathode active materials (CAMs) has come to a limit, focus shifted modification morphological aspects. However, new methodologies for quantification characteristics such as particle size, cracking, and surface area change are needed. A previously reported impedance-based method allows monitoring c...

A compact, real-time PCR instrument was developed for rapid, multiplex analysis of nucleic acids in an inexpensive, portable format. The instrument consists of a notebook computer, two reaction modules with integrated optics for four-color fluorescence detection, batteries, and a battery-charging system. The instrument weighs 3.3 kg, measures 26 x 22 x 7.5 cm, and can run continuously on the in...