stem cell therapy has been applied for the treatment of degenerative diseases encompassing corneal disorders.this consideration is regarding the unique characteristics of stem cells including self-renewal and pluripotency. some corneal diseases result from deficiencies in the limbal region. in these conditions, limbal stem cell therapy is considered in which limbal stem cells are cultured in vi...

PURPOSE To report the outcomes of allograft limbal stem cell transplantation for recurrent gelatinous drop-like corneal dystrophy (GDLD). METHODS In this non-comparative interventional case series, 4 eyes of 3 consecutive patients with recurrent GDLD underwent allograft limbal stem cell transplantation. Two eyes underwent concomitant penetrating keratoplasty while the other two underwent simu...

The most difficult part in ocular surface reconstruction for total limbal stem cell deficiency is restoring a healthy and stable corneal epithelium. Recently, there has been a shift toward a cellular-based transplantation that eliminates the need for harvesting large amounts of limbal tissues from a living donor. The autologous oral epithelium can be used as a source for ocular surface reconstr...

Transplantation of ex vivo cultured limbal epithelial cells is proven effective in restoring limbal stem cell deficiency. The present study aimed to investigate the promoting effect of Y-27632 on limbal epithelial cell proliferation. Limbal explants isolated from human donor eyes were expanded three weeks on culture dishes and outgrowth of epithelial cells was subsequently subcultured for in vi...

The integrity and normal function of the corneal epithelium are essential for maintaining the cornea's transparency and vision. The existence of a cell population with progenitor characteristics in the limbus maintains a dynamic of constant epithelial repair and renewal. Currently, cell-based therapies for bio-replacement, such as cultured limbal epithelial transplantation and cultured oral muc...

Regenerative medicine for the cornea provides a novel treatment strategy for patients with corneal diseases instead of conventional keratoplasty. Limbal transplantation has been performed in patients with a limbal stem cell deficiency. This procedure requires long-term immunosuppression that involves high risks of serious eye and systemic complications, including infection, glaucoma, and liver ...

Total bilateral Limbal Stem Cell Deficiency is a pathologic condition of the ocular surface due to loss corneal stem cells. Cultivated oral mucosa epithelial transplantation (COMET) only autologous successful treatment for this pathology in clinical application, although abnormal peripheric vascularization often occurs. Properly characterizing regenerated needed reliable follow-up. So far, univ...