purpose: to report the outcomes of conjunctival-limbal autograft (clau) in patients with unilateral total limbal stem cell deficiency (lscd) emphasizing surgical problems, complications, and their management. methods: in this prospective interventional case series, clau combined with amniotic membrane transplantation as a graft was performed on 26 patients with unilateral total lscd due to chem...

stem cells located at the limbus ,  are the ultimate source for regeneration of the corneal epithelium in the normal and traumatized states.  when limbal stem cells are dysfunctional or deficient, limbal stem cell deficiency (lscd) develops. its surgical management depends on laterality and severity of corneal-limbal involvement. conventional methods of stem cell transplantation are conjunctiva...

we are describing a new surgical approach in the management of advanced ocular surface cicatrization through a single procedure conjunctival-limbal-corneal allograft in which the external eye is resurfaced it involves a lamellar corneolimbal keratoplasty, 360° limbal stem cell transfer (on a “bipedicle” conjunctival-corneal carrier), and conjunctival tissue transplantation - all in a single con...

purpose: to report the early results of transplantation of autologous limbal stem cell cultivated on amniotic membrane in total limbal stem cell deficiency. methods: four eye of 4 patients with total unilateral stem cell deficiency secondary to severe chemical or thermal burn were included. stem cell deficiency was confirmed with impression cytology in all cases. under topical anesthesia, a sma...

purpose: to investigate the efficacy of limbal-conjunctival autografting technique in patients with primary pterygium methods: fifteen eyes of 15 patients with primary pterygium underwent surgery for the removal of pterygium with limbal-conjunctival autograft. after the pterygium excision, the limbal portion of the graft was oriented and sutured to the limbus at the recipient bed with the epith...

purpose: to report the clinical findings and compare the outcomes of different surgical techniques evolved over time in a large series of patients with delayed-onset mustard gas keratitis (mgk). methods: this interventional retrospective case series includes patients with complications of delayed mgk. medical and surgical interventions to address dry eye, limbal ischemia and limbal stem cell de...

INTRODUCTION Chemical burns of the eyes are one of the important causes of visual disability. Chemical burns particularly alkali burn may lead to gross limbal stem cell deficiency. Amniotic membrane transplantation with limbal graft is a good method for ocular surface reconstruction. CASE A 39 years male presented to Biratnagar Eye Hospital after 5 months of chemical injury with redness, pain...

Severe damage to cell repair mechanisms of the limbal region can lead to many disorders such as vascularized conjunctivalization, keratinization, corneal scarring, and corneal opacification, collectively described as limbal stem cell deficiency (LSCD). Limbal stem cell deficiency may occur as a result of depletion of stem cells or destruction of their stromal niche. In such cases, apart from co...

PURPOSE To correlate a biomicroscopic evaluation, an in vivo confocal microscopy examination, and impression cytologic findings of the corneal center and sclerocorneal limbus after cultured limbal stem cell transplantation and to test the effectiveness of in vivo confocal microscopy as a diagnostic procedure in ocular surface cell therapy reconstructive surgery. METHODS Six eyes of six patien...