RAC3 is a transcription coactivator, usually overexpressed in several tumors and required to maintain the pluripotency in normal stem cells. In this work we studied the association between RAC3 overexpression on cancer cell stemness and the capacity of this protein to induce cancer stem properties in non tumoral cells. We performed in vitro and in vivo experiments using two strategies: by overe...

Intra-arterial metabolic radiotherapy (using lipiodol labelled with iodine-131 or rhenium-188) is a therapeutic approach that can be used for the treatment of hepatocellular carcinomas (HCC). We propose a detailed description of the tumoral model using the N1-S1 cell line as well as a technique for intra-arterial injection of radiolabelled lipiodol in order to undertake preclinical studies nece...

Breast cancer tissues include carcinoma cells and stromal cells, intra-tumoral stroma that consists of different types cells. For this point, cell-cell interaction communication have a potential role in progression. Mono-cell culture is used for treatment approaches. However, can not be evaluated on mono-culture So, co-culture models provide low-cost screening to determine cell proliferation dr...

Angiogenesis and lymphangiogenesis are involved in tumoral growth and metastatic spread. There is little information on angiogenesis and no available data on lymphangiogenesis in parathyroid glands (PTG). Using immunohistochemistry for CD34, LYVE-1 (specific markers for vascular and lymphatic endothelium, respectively), vascular endothelial growth factor (VEGF)-A, VEGF-C, and fibroblast growth ...

The molecular mechanisms that regulate the formation of the lymphatic vascular system remain poorly characterized. Whereas studies in embryonic stem (ES) cells have provided major new insights into the mechanisms of blood vessel formation, the development of lymphatic endothelium has not been previously observed. We established embryoid bodies (EBs) from murine ES cells in the presence or absen...

Bioactive derivatives of cholesterol have been demonstrated to regulate immune cell function and migration, the tumor microenvironment (TME), consequently progression. Ch25h, a key enzyme involved in metabolism 25- hydroxycholesterol(25-HC) production, was positively correlated with overall survival melanoma, breast cancer colon patients. High Ch25h expression is good response immunotherapy mel...

Background and aims: Infectious diseases are disorders caused by organisms such as bacteria, viruses, fungi or parasites .The Th9 subset develops in response to combined signals from TGF-b and IL-4 among a cacophony of other cytokines in an extracellular milieu. T helper 9 (Th9) cells,  as a novel CD4 T cell subset, seem to play a complex role in the outcome of specific immune responses. In thi...

The lymphatic vasculature is important for the regulation of tissue fluid homeostasis, immune response, and lipid absorption, and the development of in vitro models should allow for a better understanding of the mechanisms regulating lymphatic vascular growth, repair, and function. Here we report isolation and characterization of lymphatic endothelial cells from human intestine and show that in...

AIM The authors assessed the influence of preoperative radiotherapy on autophagy process using a quantitative assessment of LC3 expression on both normal and tumoral colorectal tissues. MATERIALS AND METHODS Normal and malignant tissue samples were taken from 50 patients that underwent surgery for colorectal adenocarcinoma of which 11 received preoperative radiotherapy. Tissue samples were in...