The diagnosis of smear-negative and culture-negative patients with active tuberculosis (TB) is challenging. The detection of Mycobacterium tuberculosis-specific antibodies in human sera has been an important diagnostic aid. However, detection of antibody responses to a single antigen usually has a low sensitivity for diagnosis of TB. In this study, humoral immune responses against recombinant M...

The ability of tuberculosis patients to recognize Mycobacterium vaccae-specific antigens before starting chemotherapy and according to disease severity was analyzed. We report that the M. vaccae cell wall skeleton fraction triggers more enhanced cytokine production than the whole bacterium. Moreover, a tendency was observed for a lower gamma interferon/interleukin-10 ratio in patients with cavi...

tuberculosis (tb) is an important health problem in developing countries, with varying clinical presentations depending on the organs/systems involved. to study the spectrum of clinical and paraclinical aspects of extra pulmonary tb in children suffering from pulmonary tb. this study has been carried out on 65 children with tuberculosis, admitted in tb wards of national research institute of tu...

The seroreactivity of a cocktail of purified mycobacterial excretory–secretory (ES) antigens ES-31, ES-41 and ES43 was assessed by ELISA and immunoblotting in patients with pulmonary tuberculosis. The ES-31 antigen was isolated by affinity chromatography and ES-41 and ES-43 were isolated by fast protein liquid chromatography from Mycobacterium tuberculosis H37Ra culture filtrate. Seven of 27 pu...

The diagnosis of pulmonary tuberculosis and tuberculous pleurisy standard methods is difficult and slow. Now, high hopes to cells of T cells secreting interferon-gamma in response to antigens of Mycobacterium tuberculosis. Use of the immune response to TB diagnosis. A systematic review was performed of latest publications. Interferon-γ release assays (IGRA) on peripheral blood mononuclear cells...

Recent advances in molecular and genomic techniques have facilitated research on several aspects of mycobacteriology, such as diagnosis and the identification of new vaccines and therapeutic targets for various diseases, including tuberculosis. The aim of this review was to analyze the implications of advances in molecular and genomic techniques on the development of new vaccines for tuberculos...