We welcome the short report of Suntornsut and others, which reminds us of melioidosis, an emerging and potentially fatal infection in Southeast Asia and other regions. Burkholderia pseudomallei might provoke a large variety of symptoms and mimic tuberculosis (TB). Misdiagnosis may delay initiation of adequate treatment and increase the risk for a severe outcome. Among 118 enrolled patients with...

Background and Objective: Tuberculosis is still a major health problem, involving about 1/3 of the world´s population. Diagnosis is difficult when we only use Ziehl-Neelson staining. Many cases may be missed. A more rapid and sensitive diagnostic method is necessary. PCR may be helpful. The aim of this study was to compare PCR, Zieh-Neelsen staining and histopathologic findings in diagnosis of ...

A total of 320 faecal specimens obtained from 295 patients (192 children and 103 adults) with diarrhoea and 47 faecal specimens from healthy adults were screened for Cryptosporidium oocysts. Faecal specimens were first screened by modified Ziehl Neelsen stain and specimens with oocysts were confirmed by direct immunofluorescence technique. Cryptosporidium oocysts were detected in 4 children but...

The principles and techniques involved in fluorescence microscopy as related to the demonstration of acid-fast bacilli are discussed. The auramine-rhodamine staining method, for both tissue and bacteriological preparations, is described as well as the necessary equipment for both microscopy and photomicroscopy. Fluorescence staining is no more difficult nor time consuming than a Ziehl-Neelsen s...

Three hundred stool samples for routine ova and parasite examination were examined as saline and iodine wet preparations, the results were then compared with the Zeihl-Neelsen-modified Acid Fast staining (MAFS), as the oocysts are acid fast in character. Examination with saline preparation was unable to detect any oocyst, while iodine preparation yielded three oocyst and stained smears with MAF...