results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...

evidences from many studies suggested a polymerase chain reaction (pcr) as a valuable method for diagnosing infectious disease in the transplant recipients. we used this method for detection of toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or kidney transplantation. dna of both infectious agents were detected using two separate sets of nested pr...

Bovine viral diarrhoea virus (BVDV) is an important pathogen of dairy cattle. In this study, bulk milk samples representing a total of 4105 milking cows, from 18 dairy cattle herds in the suburb of Mashhad- Iran, were tested for presence of BVDV by the use of a nested reverse transcription polymerase chain reaction (Nested RT- PCR) assay. Non of the cows in the herds had been vaccinated against...

background: human enteroviruses (evs) may have a role as a possible risk factor in the pathogenesis of mi. the aim of this study was to evaluate the presence of enterovirus genomic rna in peripheral blood samples of patients with acute myocardial infarction (mi). methods: we investigated the presence of enterovirus genomic rna in the peripheral blood of 115 patients with acute mi hospitalized i...

background: vasopressin type 2 receptor (v2r) plays an important role in the water reabsorption in the kidney collecting ducts. v2r is a g protein coupled receptor (gpcr) and the triplet of amino acids aspartate-arginine-histidine (drh) in this receptor might significantly influence its activity similar to other gpcr. however, the role of this motif has not been fully confirmed. therefore, the ...