determination of the accurate time of egg stripping after ovulation has been regarded as one of the most important factors in the successful reproduction of caspian brown trout(salmo trutta caspius). to determine the best time for egg stripping after ovulation and over- ripening of the oocyte in the caspian brown trout , the eggs of fish were retained in the coelomic cavity of females at 7 ± 6°...

The ovulatory process is tightly regulated by endocrine as well as paracrine factors. In the periovulatory period, extensive remodeling of the follicle wall occurs to allow the extrusion of the oocyte and accompanying cumulus granulosa cells. Growth differentiation factor-9 (GDF-9) and bone morphogenetic protein-15 (BMP-15) are secreted members of the TGFbeta superfamily that are expressed begi...

Thin slices of human ovarian cortex were evaluated following cryopreservation in 1,2-propanediol (PROH)/sucrose under various conditions. Following rapid thawing, 1 microm sections were assessed by light microscopy and oocyte abnormalities were further examined by electron microscopy. Follicles (n = 503) were predominantly primordial (91%), with no follicles larger than the proliferating primar...

Abstract Study question Which confounders (sperm quality, oocyte dysmorphism, culture time, images pre or post-ICSI, age) affect the ability of AI to predict blastulation based on images? Summary answer Sperm post-ICSI image should be controlled for when building algorithms images. What is known already Previous studies reporting use have: (i) not accounted affecting (i.e. sperm time), and (ii)...

The eighth report of the European Society of Human Reproduction and Embryology PGD Consortium is presented documenting cycles collected for the calendar year 2005 and follow-up of the pregnancies and babies born until October 2006 which resulted from these cycles. For the first time, the delivery rates for each indication are presented and also the pregnancy rates for each centre are reported a...

Background: Intracytoplasmic sperm injection (ICSI) has become a useful technology to produce foals when availability of semen is limited or when in-vitro fertilization is desired, as is the need for subfertile mares. However, its application into clinical practice is challenging. The purpose of this review was to discuss some fundamental molecular aspects of oocyte maturation that should be co...

Background: The meiotic spindle plays an important role in the oocyte during chromosome alignment and separation at meiosis. The zona pellucid (ZP) is a dynamic matrix composed of filaments with the properties that might reflect the history of oocyte cytoplasmic maturation. Since, spindle and ZP in living oocytes are highly birefringent, their structures can be viewed noninvasively by using a P...

This is a retrospective study aimingto clarify the current status of preimplantation genetic diagnosis (PGD) in Japan. Our data were collected from 12 facilities between September 2004 and September 2012, and entered into a database. A majority of PGD in Japan was performed for balanced structural chromosomal abnormalities in couples with recurrent miscarriage. PGD for monogenic diseases was pe...