Bacterial microcompartments (MCPs) are sophisticated protein-based organelles used to optimize metabolic pathways. They consist of metabolic enzymes encapsulated within a protein shell, which creates an ideal environment for catalysis and facilitates the channeling of toxic/volatile intermediates to downstream enzymes. The metabolic processes that require MCPs are diverse and widely distributed...

This paper addresses the question of whether microtubule-directed transport of vesicular organelles depends on the presence of a pool of cytosolic factors, including soluble motor proteins and accessory factors. Earlier studies with squid axon organelles (Schroer et al., 1988) suggested that the presence of cytosol induces a > 20-fold increase in the number of organelles moving per unit time on...

Abstract Eukaryotic cells are complex systems compartmentalized in membrane-bound organelles. Visualization of organellar electrical activity living requires both a suitable reporter and non-invasive imaging at high spatiotemporal resolution. Here we present hVoS org , an optical method to monitor changes the membrane potential subcellular membranes. This takes advantage FRET pair consisting vo...