نتایج جستجو برای: pcdna3
تعداد نتایج: 296 فیلتر نتایج به سال:
We report a novel t(7;9)(q11;p13) translocation in 2 patients with B-cell acute lymphoblastic leukemia (B-ALL). By fluorescent in situ hybridization and 3' rapid amplification of cDNA ends, we showed that the paired box domain of PAX5 was fused with the elastin (ELN) gene. After cloning the full-length cDNA of the chimeric gene, confocal microscopy of transfected NIH3T3 cells and Burkitt lympho...
Nonobese diabetic (NOD) mice develop insulitis and diabetes through a process involving autoimmunity to the 60-kDa heat shock protein (HSP60). Treatment of NOD mice with HSP60 or with peptides derived from HSP60 inhibits this diabetogenic process. We now report that NOD diabetes can be inhibited by vaccination with a DNA construct encoding human HSP60, with the pcDNA3 empty vector, or with an o...
BACKGROUND AND THE PURPOSE OF THE STUDY Angiogenesis is an important process in physiology and disease pathogenesis and is controlled in a healthy body by a number of stimulatory and inhibitory factors. The aim of this study was to determine the effect of antisense transcript on the sense transcript of the endothelial growth factor (EGF) gene in bacterial system as an approach for the gene regu...
علی رغم استفاده از واکسن ب.ث.ژ از سال 1921 تا به حال، هنوز بیماری سل به عنوان دومین عامل مرگ ومیر در بین بیماریهای عفونی محسوب می گردد. با افزایش مقاومت عامل مولد سل (mycobacterium tuberculosis) به آنتی بیوتیک های معمول و وسیع الطیف و همچنین با ظهور hiv و عفونت این ویروس با مایکوباکتریوم توبرکولوزیس، مقابله با این بیماری بیشتر به چالش کشیده شده است. شناخت آنتی ژنهای موثر در ایمنی زایی محافظتی م...
Melanoma differentiation‑associated‑7 (mda‑7)/interleukin‑24 (IL‑24), a unique cytokine‑tumor suppressor, exerts tumor‑selective killing activity in numerous types of cancer cell. Although eukaryotically and prokaryotically expressed recombinant human (rh)IL‑24 proteins have been previously shown to produce potent antitumor effects, to the best of our knowledge, no side‑by‑side study has been c...
BACKGROUND Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided adaptive immune systems are found in prokaryotes to defend cells from foreign DNA. CRISPR Cas9 systems have been modified and employed as genome editing tools in wide ranging organisms. Here, we provide a detailed protocol to truncate genes in mammalian cells using CRISPR Cas9 editing. We describe custom don...
toxoplasmosis is a common and widespread infection in humans and many other species of warm–blooded animals. toxoplasma gondii-derived heat shock protein 70 (hsp70) may play an important role in the virulence of toxoplasma gondii (t. gondii). in the present study, t. gondii hsp70 was amplified by polymerase chain reaction (pcr) from the dna of the t. gondiitachyzoite rh strain through the use o...
پیش زمینه و هدف: علی رغم استفاده از واکسن ب.ث.ژ از سال 1921 تابه حال، هنوز بیماری سل به عنوان دومین عامل مرگ ومیر در بین بیماری های عفونی محسوب می گردد. با افزایش مقاومت عامل مولد سل (مایکوباکتریوم توبرکولوزیس) به آنتی بیوتیک های معمول و وسیع الطیف و همچنین با ظهور hiv و عفونت این ویروس با مایکوباکتریوم توبرکولوزیس، مقابله با این بیماری بیشتر به چالش کشیده شده است. شناخت آنتی ژن های مؤثر در ایم...
Abstract Background and Objective: The M2 gene expressing the conserved protein in influenza virus can be used to make a single-dose vaccine with permanent immunity. Material and Methods: The mice were allocated to one case group immunized with pcDNA3-M2 and two control groups with pcDNA and PBS, in three dozes with interval of two weeks. Two weeks after the last injection, Cellular imm...
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