cantharis q has a long history in both folk and traditional medicine being used as an aphrodisiac. it is a crude alcoholic extract of blister beetle lytta vesicatoria commonly known as spanish fly having cantharidin, a venomous substance present in it. the aim of the present study was to evaluate the clinical application of cantharis q by assessing its effect on the vital organ, liver, for a sh...

immunohistochemistry.Human turbinates were obtained after turbinectomy from 6 patients with nasal obstruction refractory to medical therapy. Total RNA was isolated from human nasal mucosa, and CysLT2 receptor mRNA was detected in these tissues by using reverse transcriptase-PCR analysis. To identify the cells expressing CysLT2 receptor protein, double immunostaining was performed using anti-Cys...

immunohistochemistry.Human turbinates were obtained after turbinectomy from 6 patients with nasal obstruction refractory to medical therapy. Total RNA was isolated from human nasal mucosa, and CysLT2 receptor mRNA was detected in these tissues by using reverse transcriptase-PCR analysis. To identify the cells expressing CysLT2 receptor protein, double immunostaining was performed using anti-Cys...

M uch interest has been focused in nucleic acid isolation from tissue samples stored for long periods and their utilization on molecular biomarkers research. However, sample fixation induces chemical modifications in macromolecules (1) that result in a laborious and difficult protocol to extract nucleic acid for molecular analysis. In this context, the article recently published by Kizys and co...

Gene expression studies require analysis of RNA, but isolation of total RNA from very small samples by traditional methods can be difficult and inefficient. The Absolutely RNA microprep kit provides a convenient method for isolating total RNA from small numbers of cells such as those harvested by laser capture microdissection (LCM). The protocol includes binding of RNA to a solid support, thus ...

Jerusalem artichoke (Helianthus tuberosus L.) is an important tuber crop. However, Jerusalem artichoke seeds contain high levels of starch and lipid, making the extraction of high-quality RNA extremely difficult and the gene expression analysis challenging. This study was aimed to improve existing methods for extracting total RNA from Jerusalem artichoke dry seeds and to assess the applicabilit...

The preservation of banana fruit is vital to ensure ripening quality during the transportation process. can be defined using gene expression marker, which needs high-quality RNA. However, when access laboratory difficult, long-distance transportation, lack liquid nitrogen or refrigerator, we need an alternative for methods, so sample and extracted RNA are still in excellent conditions. Aim our ...