Sugar transport by some permeases in Escherichia coli is allosterically regulated by the phosphorylation state of the intracellular regulatory protein, enzyme IIAglc of the phosphoenolpyruvate:sugar phosphotransferase system. A sensitive radiochemical assay for the interaction of enzyme IIAglc with membrane-associated lactose permease was used to characterize the binding reaction. The binding i...

Lactose intolerance has been reviewed in published statements by the Protein Advisory Group of the United Nations System,’ the Food and Nutrition Board of the National Research Council,2 and the American Academy of Pediatrics, Committee on Nutrition.’ Each of these statements deals principally with the advisability of encouraging population groups with a high rate of lactose intolerance to cons...

The double mutant, Val-177/Asn-322, was investigated with regard to its ability to transport H+ and galactosides. In downhill lactose transport assays, the wild-type strain had a Km value for lactose uptake of 0.9 mM and a Vmax of 0.65 mumol lactose/min.mg protein while the mutant had a significantly higher Km value of 1.9 mM but a similar Vmax of 0.49 mumol/min.mg protein. In spite of its mode...

One hundred eighty 3-wk-old pigs (initial weight 4.8 kg) were utilized in a 35-d growth trial to determine the effects of the carbohydrate and(or) protein fraction of dried whey on pig performance. In addition, 30, 3-wk-old pigs (initial weight 4.9 kg) were used in two digestion trials to study the effects of the whey fraction on nutrient digestibility. Dietary treatments included a corn-soybea...

This study compared the composition, yield, and freezing point of buffalo colostrum milk collected on 30th, 60th, 90th day lactation from purebred Murrah its crosses with Philippine Carabao. A total 133 samples were 36 buffaloes (20 16 “Murrah x Carabao” crosses) evaluated for fat, protein, lactose content, solids non-fat (SNF), solids, point. Colostrum contained significantly (p<0.05) more ...

Valorization of by-products in the dairy industry as a strategy to promote circular economies has become priority around globe. Cheese whey and its derivates from filtration technologies offer source valuable molecules such proteins, fat, lactose, minerals. For this study, after protein concentration recovery through ultrafiltration, lactose remaining permeate was furtherly concentrated with na...

Yogurt has been one of the leading consumption products fermented milk for centuries and many positive effects in terms human health. However, yogurt is sometimes a problem individuals with lactose intolerance. It known that activated carbon ensures removal heavy metals from body by adsorbing, slows down growth Escherichia coli Staphylococcus aureus. In this study, on formation were investigate...

BACKGROUND Lactose, as the primary osmotic component in milk, is the major determinant of milk volume. Glucose is the primary precursor of lactose. However, the effect of glucose on lactose synthesis in dairy cow mammary glands and the mechanism governing this process are poorly understood. RESULTS Here we showed that glucose has the ability to induce lactose synthesis in dairy cow mammary ep...