The level of phosphorylation of any protein depends on the relative activities of protein kinases and protein phosphatases (I), but the latter enzymes have received far less attention, perhaps because of the difficulty in obtaining a well defined substrate before they can be studied. Nevertheless, information about, protein serine/threonine phosphatases has increased enormously over the past fe...

Genistein and bromotetramisole (Br-t) strongly activate cystic fibrosis transmembrane conductance regulator (CFTR; ABCC7) chloride channels on Chinese hamster ovary cells and human airway epithelial cells. We have examined the possible role of phosphatases in stimulation by these drugs using patch-clamp and biochemical methods. Genistein inhibited the spontaneous rundown of channel activity tha...

As a result of aging process, the state human body homeostasis undergoes changes. In tooth-jaw system, such changes lead to shift balance in remodeling-demineralization processes. Their intensity and dynamics depend on properties oral fluid, which plays an important role for ensuring physiological processes cavity. The purpose work was clinical assessment dental status, examination calcium home...

Phosphatases are crucial enzymes in health and disease, but the knowledge of their biological roles is still limited. Identifying substrates continues to be a great challenge. To support the research on phosphatase-kinase-substrate networks we present here an update on the human DEPhOsphorylation Database: DEPOD (http://www.depod.org or http://www.koehn.embl.de/depod). DEPOD is a manually curat...

The reversible phosphorylation of proteins on serine, threonine, and tyrosine residues represents a fundamental strategy used by eukaryotic organisms to regulate a host of biological functions, including DNA replication, cell cycle progression, energy metabolism, and cell growth and differentiation. Levels of cellular protein phosphorylation are modulated both by protein kinases and phosphatase...

Protein phosphorylation appears to be a universal mechanism of protein regulation. Genomics has provided the means to compile inventories of protein phosphatases across a wide selection of organisms and this has supplied insights into the evolution of this group of enzymes. Protein phosphatases evolved independently several times yielding the groups we observe today. Starting from a core cataly...

The effect of theta pulse stimulation (TPS) on pentylenetetrazol (PTZ)-induced long-term potentiation of population spikes (PS) was studied in the hippocampal CA1 in vitro. A transient PTZ application produced a long-lasting enhancement of PS amplitude. A 3-min episode of TPS delivered at a higher intensity produced complete reversal of the PTZ potentiation when delivered during the last minute...