Fourier-transform infrared spectroscopy is a powerful and versatile tool to investigate the structure and dynamics of proteins in solution. The intrinsically low extinction coefficient of the amide I mode, the main structure-related oscillator, together with the high infrared absorptivity of aqueous media, requires that proteins are studied at high concentrations (>10 mg L(-1)). This may repres...

The pyruvate-to-lactate assay for determining lactate dehydrogenase (EC 1.1.1.27) can now yield linearity equal to or better than that obtained by the lactate-to-pyruvate assay. In addition, there are significant advantages to the pyruvate-to-lactate reaction: (a) a greater change in absorbance per unit time, which allows more accurate spectrophotometric readout; (b) lower reactant concentratio...

The rotational signatures of methyl pyruvate as an impurity have been accidentally found and its rotational spectrum was assigned while recording the microwave spectrum of a 98% Sigma-Aldrich sample of methyl (S)-(-)-lactate with the pulsed jet Fourier transform microwave technique.

The original version of this article contained an error in testing the kinetic parameters of CgNal towards pyruvate, in which the concentration of ManNAc (50 mM) was not in excess. This error was corrected by re-running the assay in the presence of excessive ManNAc (180 mM). The corrected kinetic parameters of CgNal towards pyruvate are shown in Table 1. These changes do not change the conclusi...

enz y matic-fluorimetric-continuous-flow methods for the measurement of glucose, lactate, pyruvate, alanine, glycerol and 3-hydroxybutyrate in human blood

The "L"-type pyruvate kinase from rabbit liver does not catalyse exchange between phosphoenol[1-14C]pyruvate and pyruvate at either pH 8.5 or 6.2. Spectrophotometric experiments at pH 8.5 and 6;2 and gel-filtration experiments with [32P]phosphoenolpyruvate at pH 8,5 also fail to demonstrate phosphoenzyme formation. It is concluded that it is very unlikely that the enzyme has a phosphoenzyme mec...

normal and fasting conditions. Fig. 3 LDH activity with or without 5FU under normal and fasting conditions measured by absorption spectroscopy. Fig. 4 Survival rate of the cells. Fig.1 The spectrum and fitting curves of NMR measurements. Evaluation of the Early Metabolic Response induced by 5-Fluorouracil on Mouse Mammary Cancer Cells under fasting conditions using Hyperpolarized 13C-labeled Py...

A new tryptophan catabolic pathway is characterized from Burkholderia cepacia J2315. In this pathway, tryptophan is converted to 2-amino-3-carboxymuconate semialdehyde, which is enzymatically degraded to pyruvate and acetate via the intermediates 2-aminomuconate and 4-oxalocrotonate. This pathway differs from the proposed mammalian pathway which converts 2-aminomuconate to 2-ketoadipate and, ul...

An enzyme system in rat liver catalyzes the decomposition of mercaptopyruvate to pyruvate and sulfur (1). At the optimal pH of this system, this reaction has been shown to be a transsulfuration (2). Acceptors of the sulfur include cyanide (2), sulfite and sulfinates (3). In the absence of an acceptor, the sulfur is fixed in a reactive form which decomposes to free sulfur at lower pH. This repor...

We have developed a practical synthesis of unique nucleoside derivatives via TiCl(4) promoted multicomponent reaction of optically active dihydrofuran, ethyl pyruvate/glyoxylate, and a TMS protected nucleobase in a single-pot operation.