Real-time PCR (qPCR) was positive in 72/150 (48%) blood samples of newborns with congenital toxoplasmosis. Among infants with active retinochoroiditis, 68% had positive qPCR results, while positivity was 29% (P=0.009) in the absence of ocular involvement. Positive qPCR was associated with the presence of retinochoroidal lesions, with an odds ratio of 2.8.

Background: Epstein-Barr virus (EBV)-associated post-transplant lymphoproliferative disorder (EBV-PTLD) is a known and serious condition for the hematopoietic stem cell transplantation. This study evaluated the prevalence of EBV in umbilical cord samples of healthy pregnant women in Baghdad, Iraq.  Methods: 800 umbilical cord blood (UCB) samples were collected from healthy pregnant women. The ...

The Eco Real-Time PCR system puts powerful qPCR applications within reach of individual researchers. Large, expensive thermal cyclers that take up an entire workspace are replaced with an affordable, compact system that fits easily on any lab bench (Figure 1). Delivering unsurpassed data quality in less than 40 minutes for 40-cycle runs, the Eco system revolutionizes qPCR accessibility for both...

The definitive diagnosis ability of IgM ELISA, nested RT-PCR and real-time quantitative PCR (qPCR) was evaluated for Chikungunya diagnosis using 180 clinical samples. Real-time qPCR showed a higher sensitivity (88.3%) for Chikungunya diagnosis in the early stages of infection, while IgM ELISA proved sensitive for the late stages of illness (81.8%). The results suggest that the application of bo...

abstract background: cancers are the second most common cause of non-accidental deaths in iran, after cardiovascular mortality. although most cases of esophageal squamous cell carcinoma (escc) in the usa and western populations have been attributed to high levels of exposure to tobacco and alcohol, but in iranian populations the other risk factors especially infectious agents have been postulat...

The Eco Real-Time PCR system puts powerful qPCR applications within reach of individual researchers. Large, expensive thermal cyclers that take up an entire workspace are replaced with an affordable, compact system that fits easily on any lab bench (Figure 1). Delivering unsurpassed data quality in less than 40 minutes for 40-cycle runs, the Eco system revolutionizes qPCR accessibility for both...