Fundamental questions in bacterial gene regulation concern how multiple regulatory proteins interact with the transcription apparatus at a single promoter and what are the roles of protein contacts with RNA polymerase and changes in DNA conformation. Transcription of the Escherichia coli uhpT gene, encoding the inducible sugar phosphate transporter, is dependent on the response regulator UhpA a...

Information on the genetic diversity of pathogenic bacteria causing plant disease is rare to find. This study aims identify and classify disease-causing pathogens in several ornamental plants molecularly. Sixteen bacterial strains were isolated from carnation, chrysanthemum, kalanchoe. All grouped D. dianthicola although carnation kalanchoe gave different results identified strains. The differe...

High-quality mRNA extraction is essential for gene expression assays. In this study, we developed a rapid method (20 min) named FACTS the of intact RNA from Pseudomonas aeruginosa and compared its performance to established techniques like RNAsnap CiAR. The integrity, yield, purity presence residual genomic DNA were adopted as assessment criteria. Multiple assays integrity applied, including Ag...

The occurrence of the cyanobacterial toxin cylindrospermopsin (CYN) in freshwater reservoirs is a common phenomenon. However, the biodegradation of this toxin in environmental samples has been observed only occasionally. In this work the biodegradation ability of cylindrospermopsin was investigated based on isolates from lakes with previous cyanotoxin history. Bacterial strains were identified ...