the effect of sub culturing frequencies and different cytokinins (benzyl amino purine (bap), 2-isopentenyl adenine (2ip) on genetic stability of micropropagated shoots from olive plant were investigated by using physiological traits and random amplified polymorphic dna (rapd) markers. axillary buds of the olive (cv. dezful) plants were cultured and subcultured in dkw medium, supplemented with b...

it is important to identify the genetic diversity and phylogenic relationships for achieving desirable citrus cultivars. in present investigation rapd markers were used to determine genetic diversity among 29 citrus genotypes. from 19 used primers, 248 polymorphic bands were amplified. primers opa-07 and opa-05 (with 19 and 8 amplified bands), produced maximum and minimum polymorphic bands, res...

the genetic diversity of 40 tobacco (nicotiana tabacum l.) cultivars was analyzed using 12 issr primers. thecultivars were evaluated for 14 traits using a simple lattice design (7×7) with two replications. of 149 amplified bands, 142 (95.3%) were polymorphic. maximum and minimum bands were generated by primers ubc825 (17 bands and 94.11% polymorphic) and ubc824 (7 bands and 100% polymorphic), r...

  Random amplified polymorphic DNA (RAPD) markers were used to estimate genetic relationships between two seasonal immigrant forms (namely fall-run and spring-run) of Caspian trout Salmo trutta caspius. In this regard, 62 fin tissue samples were collected from southern parts of the Caspian Sea basin and examined with 16 oligodecamer primers to asses their genetic diversity as well as probable s...

three iranian native strains and three japanese commercial lines of the silkworm bombyx mori were analyzed using amplified fragment length polymorphism (aflp) markers. a set of ten psti/taqi primer combinations amplified a total of 322 bands out of which 251 (%78) were polymorphic. estimates of nei’s gene diversity for all loci in individual strains and commercial lines indicated a higher degre...

Cashmere is only produced from Maraz (Kurdi) goat breed in Iraqi Kurdistan Region. The objective of this study was to assess the genetic diversity of different Maraz color types, black goat and Shami breeds using random amplified polymorphic DNA (RAPD) markers. Fourteen primers were used and nine out of them were selected based on their number of bands (NB) and polymorphic characteristics. Thes...

Centaurea amaena is an endemic and endangered species listed as CR (critically endangered) in Turkey. ISSR markers were used to detect the level of genetic diversity two natural populations C. amaena. A total 50 primers 13 producing polymorphic reproducible products selected. These yielded 102 amplified discernible loci, which 80 (78%) polymorphic. high was detected both at population levels; e...

Chamomile (Matricaria chamomilla), an important medicinal plant belonging to the Asteraceae family, has a wide distribution in Iran and other parts of the world. The medicinal and pharmacological effects of chamomile are mainly associated with its essential oil content and it is widely used in food, cosmetics and pharmaceutical industries. Despite its wide geographical distribution in Iran, lit...

Abstract Randomly amplified polymorphic DNA (RAPD) analysis was performed to infer the magnitude and pattern of genetic differentiation among boll weevil populations from eighteen locations across eight US states and north-east Mexico. Sixty-seven reproducible bands from six random primers were analysed for genetic variation within and between weevil populations. Genetic and geographical distan...