The first experiments on hydrogen transfer in the thymidylate synthetase reaction (Equation 1) were reported by Friedkin (2, 3). Borohydride labeled with tritium was used to reduce folic acid to tritium-labeled tetrahydrofolate which was then treated with formaldehyde to yield the 5, lo-methylene derivative. When the latter compound was used as substrate for thymidylate synthetase (prepared fro...

This review briefly describes techniques and basic results of experimental investigations in mice and rats on metabolism, dosimetry, and radiobiological effects of tritium oxide and some tritiated biogenic compounds (glucose, amino acids, and nucleosides) during the last 10 to 15 years in Russia. The content of water in tissue cells of mammals is shown to be 15 to 40% less than in whole tissue....

When highly purified cobamide-dependent ribonucleotide reductase from Lacfobacillus leichmannii is incubated with synthetically prepared 5,6-dimethylbenzimidazolylcobamide 5’-deoxyadenosyl coenzyme containing tritium attached to carbon atom 5’ of the deoxyadenosyl moiety (DBCC-5’-3H), tritium is transferred from DBCC-5’-3H to Hz0 in a reaction requiring substrate, enzyme, and dithiol reductant....

Accurately predicting the quantity of tritium retained in plasma facing components is a key safety issue for licensing future fusion power reactors. Retention of tritium in the lattice damage caused when high energy neutrons collide with atoms in the structural material of the reactor’s plasma facing components (PFCs) is an area of ongoing experimental research at the Idaho National Laboratory ...

Tritium accumulations in each organism as organic bound form are expressed as the R value which is defined as the ratio of tritium specific activity in lyophilized organisms (ƒÊCi/gH) to that in water (ƒÊCi/gH). The maximum R values were 0.5 in diatoms, Chaetoceros gracilis, 0.5 in brine shrimps, Artamia salina, and 0.32 in Japanese killifish, Oryzias latipes under the growing condition where t...

As one of the three natural isotopes of hydrogen, tritium is ubiquitous and may potentially be present in any water or organic molecule that constitutes a biological matrix. Milk is one of the most frequently monitored foodstuffs in the vicinity of chronic release of radionuclides, as it is a very common food product and also because it integrates deposition on large areas of grass or crops at ...

Only limited data are available on tritium release from irradiated Be. Few models have been suggested for tritium release from beryllium. Simple diffusion/desorption models have been proposed, but lack the capability of accounting for important phenomena such as burst release through interconnected porosity and the effect of the Be0 impurity layer. This paper describes the application of a new ...

UNLABELLED BACKGROUND The nuclear-chemical method brought unique opportunity for synthesis of unknown and hardly available organic compounds. Presence of tritium labeling allows one-step preparation of radioactive markers for the investigation of chemical and biological processes. METHODS The ion-molecular reactions of nucleogenic phenyl cations with 4-picoline have been carried out. The p...

Three-dimensional diodes fabricated by electrochemical etching are exposed to tritium gas at pressures from 0.05 to 33 atm at room temperature to examine its power scaling performance. It is shown that the three-dimensional microporous structure overcomes the self-absorption limited saturation of beta flux at high tritium pressures. These results are contrasted against the three-dimensional dev...

The observation of significant neutron yield from gas loaded titanium samples at Frascati in April 1989 opened up an alternate pathway to the investigation of anomalous nuclear phenomena in deuterium/solid systems, complimenting the electrolytic approach. Since then atleast six different groups have successfully measured burst neutron emission from deuterated titanium shavings following the Fra...