Abstract This study was conducted to evaluate the role of oxidative stress in roots of eight wheat genotypes with different drought tolerance, subjected to drought stress. For analyzing the changes of antioxidant enzymes, native PAGE analyses of protein extract were performed. The roots of wheat seedlings showed one unambiguous isoform of superoxide dismutase (SOD) and catalase (CAT). Eight iso...

non-starch polysaccharides (e.g.: wheat based diet) which are supplemented with enzymes. could have a high potential in nutrient ingestion, digestion and absorption, however, response to the enzyme is dependent on chicks age, as well as genetic status of both the cereal and the chick. this study was conducted in a completely randomized design in 2×4 factorial arrangement to study the efficiency...

Production of a-amylase under solid-state fermentation by Bacillus cereus MTCC 1305 has been investigated using wheat bran and rice flake manufacturing waste as substrates. With wheat bran, highest enzyme production expressed as units per mass of dry substrate ((94±2) U/g) was observed. Production parameters were optimized as inoculum size 10 % (volume per mass) and substrate:moisture ratio 1:1...

Bioethanol production from lignocellulosic raw materials involves process steps like pre-treatment, enzymatic hydrolysis, fermentation and distillation. In this study, wheat straw was explored as feedstock for on-site cellulase production by T. reesei 3EMS35 mutant, and as a substrate for second generation bioethanol production from baker yeast. Scanning electron microscopy (SEM) and X-ray diff...

Agro-industrial wastes such as sugarcane bagasse, wheat bran, rice bran, corn cob and wheat straw are cheapest and abundantly available natural carbon sources. The present study was aimed to production of amylase and xylanase simultaneously using agro-industrial waste as the sole carbon source. Seven thermophilic strains of actinomycete were isolated from the mushroom compost. Among of these, s...

BACKGROUND Improving the hydrolytic performance of hemicellulases on lignocellulosic biomass is of considerable importance for second-generation biorefining. To address this problem, and also to gain greater understanding of structure-function relationships, especially related to xylanase action on complex biomass, we have implemented a combinatorial strategy to engineer the GH11 xylanase from ...

Cinnamoyl-CoA reductase (CCR) is responsible for the CoA ester to aldehyde conversion in monolignol biosynthesis, which diverts phenylpropanoid-derived metabolites into the biosynthesis of lignin. To gain a better understanding of lignin biosynthesis and its biological function, a cDNA encoding CCR was identified from wheat (Triticum aestivum L.), and designated as Ta-CCR1. Phylogenetic analysi...

Exposure to the herbicide acifluorfen resulted in marked increase of glutathione S-transferase (GST) enzyme activity in wheat seedlings, primarily in shoot tissues. From the six major, constitutively expressed GST subunits found in untreated wheat shoots subunits 2 and 3 were selectively induced by acifluorfen. No new subunit could be detected. The induced subunits belong to those GST isoenzyme...

Abstract In laboratory experiments with resting and germinated seeds of spring wheat, the effect various biological preparations on activity catalase isoforms was studied. experiments, determined at pH = 5.5, 7.0, 8.0 using a phosphate buffer system (1/15 M buffer). The enzyme in dormant wheat grain that underwent post-harvest ripening during germination third, fifth, seventh days method based ...