Benzolamide (CL-11,366), a potent carbonic anhydrase inhibitor, was used to examine the influence of carbonic anhydrase inhibition on the reabsorption of chloride, sodium, and bicarbonate in the rat proximal convoluted tubule. Administration of 2 mg/kg benzolamide was associated with a decrease in the tubular fluid/plasma (TF/P) chloride ratio from 1.19+/-0.10 (SEM) to 1.06+/-0.01, and an incre...

On the basis of polyacrylamide gradient gel electrophoresis of leaf extracts from 24 species of higher plants, two main forms of carbonic anhydrase (EC 4.2.1.1) were recognized; the "dicotyledon" type and the "monocotyledon" type. More than one band of enzyme was found on gels from most species, suggesting the possibility of carbonic anhydrase isoenzymes in higher plants.

We studied the relationship between alpha- and beta-adrenergic agonists and the activity of carbonic anhydrase I and II in erythrocyte, clinical and vessel studies. Kinetic studies were performed. Adrenergic agonists increased erythrocyte carbonic anhydrase as follows: adrenaline by 75%, noradrenaline by 68%, isoprenaline by 55%, and orciprenaline by 62%. The kinetic data indicated a non-compet...

IN an investigation of the function of the enzyme carbonic anhydrase in the formation of the aqueous humour (Langham, 1958b), studies were made with a new carbonic anhydrase inhibitor, 5-acetylimino-4-methyl-A2-1, 3, 4thiadiazoline-2-sulphonamide (Neptazane, Lederle Laboratories). The results of this study indicated that Neptazane was more active than the wellknown carbonic anhydrase inhibitor ...

Carbonic anhydrase (CA) inhibitors are valuable molecules based on several therapeutic applications, including antiglaucoma activity. In the present study, inhibition of two human cytosolic carbonic anhydrase isozymes I and II with some uracil derivatives (3-9) were investigated. Compounds 3-9 showed KI values in the range of 10.83-464 μM for hCA I and of 28.88-778.5 μM against hCA II, respecti...

Ion mobility experiments coupled with electrospray ionization (ESI) were conducted to evaluate the folding states of bovine carbonic anhydrase 2 (CA2) under three different pH conditions. Collision cross-section (CCS) of the CA2 ions generated by ESI revealed the presence of six discrete conformers in the gas phase under the conditions employed in this study. The CCS of the most extended confor...

By BERT L. VALLEE, M.D., HERBERT D. LEWIS, M.D., MARK D. ALTSCHULE, M.D., AND JOHN G. GIBSON, II, M.D. I T HAS been shown that zinc is a component of the carbonic anhydrase molecule.” 2 This enzyme has been demonstrated to catalyze the reaction H,O + CO, ± H,CO, in vitro; its molecular weight is approximately half that of hemoglobin,’ and some of its other physico-chemical properties are establ...

Davenport and Fisher (1938) and Davenport (1939, 1940) have shown that there is a high concentration of carbonic anhydrase in the parietal cells and a lower concentration in the cells of the surface epithelium of the gastric mucosa of rats, cats and dogs. They expressed the belief that the enzyme is part of the mechanism which secretes hydrochloric acid. Davenport argued that carbon dioxide is ...