micrornas (mirnas) are short rna molecules which bind to target mrnas, resulting in translational repression and gene silencing and are found in all eukaryotic cells. approximately 2200 mirna genes have been reported to exist in the mammalian genome, from which over 1000 belong to the human genome. many major cellular functions such as development, differentiation, growth, and metabolism are kn...

materials and methods: in order to achieve this, recombinant plasmid harboring antisense sequences targeting both of pta and acka was assembled, after transfecting to the cells, its effects on the cell growth and acetate accumulation in the minimal media was assessed and compared with the control, the plasmid without antisense cassette, in presence and absence of iptg in escherichia coli bl21 (...

Introduction: Although various liquid, solid, and gaseous streams of wastewater treatment plants (WWTPs) have been analyzed in many studies for the presence of SARS-CoV-2 RNA, no study was found to sample and detect SARS-CoV-2 RNA in screenings and grit samples separated from primary treatment units of WWTP. Hence, this study aims to provide an experimental protocol for sampling and extracting ...

background: rna molecules play many important regulatory, catalytic and structuralroles in the cell, and rna secondary structure prediction with pseudoknots is one themost important problems in biology. an rna pseudoknot is an element of the rna secondary structure in which bases of a single-stranded loop pair with complementary basesoutside the loop. modeling these nested structures (pseudokno...

the discovery of rna interference (rnai) in recent years has revolutionized biological and medical research. rnai is the sequence-specific gene silencing triggered by double-stranded rna (dsrna). until a couple of years ago only biologists working with caenorhabditis elegans and plants could use rnai technology to gain insight into gene function……