ON THE INNER BACK COVER: NRK 49F (with endogenous Septin2‐GFP) cell was expanded and stained with antibodies against GFP, Septin7 Septin2 (cyan), alpha/beta‐tubulin (magenta). Credit: Nadja Hümpfer Helge Ewers, Freie Universität Berlin image

Background: Chlamydomonas reinhardtii is a novel recombinant eukaryotic expression system with many advantages including fast growth rate, rapid scalability, absence of human pathogens and the ability to fold and assemble complex proteins accurately, however, obstacle relatively low expression level necessitates optimizing foreign gene expression in this system. The Green Fluorescent Protein (G...

It is possible to use transgenic plants, as bioreactors, for the production of recombinant inexpensive chemicals and drug components. Transient gene expression is an appropriate alternative to stable transformation because it allows an inexpensive and rapid method for expression of recombinant proteins in plant tissues. In recent years, plant viral vectors have been increasingly developed as su...

Green fluorescent protein (GFP), together with its family of variants, is the most widely used for in vivo imaging. An investigation influence fluorine and methoxy substituents on electronic structure GFP chromophore.

objective(s):n-myc downstream regulated gene 2 (ndrg2) is a candidate gene for tumor suppression. the expression of ndrg2 is down-regulated in several tumors including lung cancer. the aim of this study was to explore the effect of ndrg2 overexpression on invasion, migration, and enzymatic activity of matrix metalloproteinase-2 (mmp-2) and -9 (mmp-9) in human lung adenocarcinoma a549 cells.  ma...

In recent years, the disorderly development of local government debt financing and platforms (GFP) in China has brought huge risks. order to control increment caused by illegal GFP, state issued a series documents. Under new economic form, how determine position, choose road, plan path market-oriented for high-quality is critical GFP. This paper committed solving key points transformation paths...