The MultiCode-PLx system (EraGen Biosciences, Inc., Madison, WI) for the detection of respiratory viruses uses an expanded genetic alphabet, multiplex PCR chemistry, and microsphere flow cytometry to rapidly detect and specifically identify 17 different respiratory viruses directly in clinical specimens. The MultiCode-PLx system was tested in parallel with direct fluorescent-antibody (DFA) stai...

1. Phosphorylate oligonucleotide probe: a. Oligonucleotide primer (5 – 7 pmoles or 60 ng) 1 μL b. dH2O 6.5 μL c. 10X kinase buffer 1.5 μL d. T4 PNK (~10 U) 1 μL e. [γ-P]ATP (7000 Ci/mmole) 2 μL 2. Stop the kinase reaction by adding 500 μL of TE (pH 7.6) 3. Add 25 μg of carrier RNA. 4. Phenol/Chloroform extract 2X 5. Ethanol precipitate with sodium acetate (pH 5.2) 6. Wash with 70% ethanol and r...

In 2011, an outbreak of illness caused by Yersinia enterocolitica O:9 in Norway was linked to ready-to-eat salad mix, an unusual vehicle for this pathogen. The outbreak illustrates the need to characterize isolates of this organism, and reinforces the need for international traceback mechanisms for fresh produce.

Protein secretion systems that mediate interbacterial competition secret a wide repertoire of antibacterial toxins. A major player in these competitions is the newly discovered bacterial type VI secretion system (T6SS). We recently found that a subset of polymorphic MIX-effectors, which are a widespread class of effectors secreted by T6SSs, are horizontally shared between marine bacteria and ar...

The theory of constraints is an approach to production planning and control that emphasizes on the constraints to increase throughput by effectively managing constraint resources. One application in theory of constraints is product mix decision. Product mix influences the performance measures in multi-product manufacturing system. This paper presents an alternative approach by using of goal pro...

This paper introduces the concept of “revocable unlinkability” for unlinkable anonymous signatures and proposes a generalized scheme that modifies the signatures to include revocable unlinkability. Revocable unlinkability provides a condition in which multiple messages signed using an unlinkable anonymous signature are unlinkable for anyone except the unlinkability revocation manager. Noteworth...

Abstract The Efforts to increase mustard production can be carried out by developing cultivation technics using a Floating Hydroponic System (FHS) with additional nutrients support plant growth. This study aims determine the best combination of AB Mix and organic fertilizer nutrient concentrations for growth yield two types varieties FHS hydroponics. used was POC NASA, which contains sufficient...

A MIX net takes a list of ciphertexts (c1, · · · , cN ) and outputs a permuted list of the plaintexts (m1, · · · ,mN ) without revealing the relationship between (c1, · · · , cN ) and (m1, · · · ,mN ). This paper first shows that the Jakobsson’s MIX net of Eurocrypt’98, which was believed to be resilient and very efficient, is broken. We next propose an efficient t-resilient MIX net with O(t) s...

“MIX-net” systems protect the privacy of participants by clouding together their transactions through cascades of third parties. Reliability and trust are therefore open issues in this literature and limit the applicability of these systems. This paper discusses how the MIX approach can be adapted to put the user at the center of the protocol and in control of it, so that each participant can t...

Lactobacillus plantarum K68 (isolated from fu-tsai) and fruit-vegetable ferment (FVF) have been tested for antidiabetic, anti-inflammatory, and antioxidant properties in a rat model of insulin resistance, induced by chronic high fat-fructose diet. Fifty rats were equally assigned into control (CON), high fat-fructose diet (HFFD), HFFD plus K68, HFFD plus FVF, and HFFD plus both K68 and FVF (MIX...