BACKGROUND AND OBJECTIVE Freezing changes sperm integrity remarkably. Cryopreservation involves cooling, freezing, and thawing and all these contribute to structural damage in sperm, resulting in reduced fertility potential. Low-level laser irradiation (LLLI) could increase energy supply to the cell and cause reactive oxygen species reduction (ROS), contributing to the restoration of oxygen con...

This study investigated two hypotheses: 1) that consistent between-boar variation in frozen semen quality exists and is genetically determined, and 2) that morphologically distinct subpopulations of spermatozoa exist within fresh boar ejaculates and that the incidence of these subpopulations is correlated with semen quality following cryopreservation. Five ejaculates were collected from each of...

Considerable scope exists for the use of AI in camel breeding programmes but the technology is not fully developed. The fertility of both fresh and liquid-stored (24 h; 4°C) dromedary camel semen diluted in either Green Buffer (GB) or INRA-96 (INRA) was assessed. Different insemination doses were investigated: 150 and 300 x 10 motile sperm for fresh sperm, and 150, 300 and 600 x 10 motile sperm...

Nonylphenol (NP), an estrogen like compound classified as an endocrine disrupter capably interfering hormonal system of numerous organisms, originates principally from the degradation of nonylphenol ethoxylates (NE) widely used in industrial, agricultural, commercial and household applications as detergents, emulsifiers, wetting and dispersing agents. Due to the extensive use of NE, NP may reac...

This study investigated two hypotheses: 1) that consistent between-boar variation in frozen semen quality exists and is genetically determined, and 2) molecular markers linked to genes controlling semen freezability can be identified using amplified restriction fragment length polymorphism (AFLP) technology. Five ejaculates were collected from each of 129 boars. Semen was diluted into a commerc...

AIM To compare the seminal attributes of neat, pre-freeze (at equilibration), and post-freeze (24 h after freezing) semen in pure and crossbred Jersey bulls. MATERIALS AND METHODS Total 36 ejaculates (3 ejaculates from each bull) were collected from 6 pure Jersey and 6 crossbred Jersey bulls and evaluated for various seminal attributes during neat, pre-freeze, and post-freeze semen. RESULTS...

Two experiments were designed to evaluate the effects of the amino acids glycine and cysteine on cryopreservation of ram spermatozoa. After primary evaluation of collected ejaculates, the semen samples were pooled and diluted 1:4 before cooling (experiment 1) and freezing (experiment 2) with Tris-Citrate-Fructose-Yolk (TCFY) extender supplemented with different concentrations of glycine and cys...

Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytom...

Hermansson, U, 2006. Studies of Canine and Feline Sperm Viability under Different Storage Procedures. With special reference to chilling, freezing, and use of zona pellucida binding assays. Doctoral thesis. ISSN 1652-6880, ISBN 91-576-7117-6 Chilling and freezing of spermatozoa permits transport and exchange of genetic material over distance. Frozen spermatozoa, which can be preserved over long...

During cryopreservation sperm encounter oxidative stress due to higher production of ROS molecules and insufficient natural antioxidant defence system. Therefore, present study was designed identify the effects various glutathione (GSH) concentrations on Indian red jungle fowl (Gallus gallus murghi) quality fertility pre-freezing post-thaw incubation hours. Semen collected from eight cocks qual...