Starch-gel electrophoresis of leukocyte alkaline phosphatases, rendered soluble by treating normal leukocytes with butanol, revealed three electrophoretic variants of the enzyme. The phosphatases in similarly prepared extracts of leukemia cells differed from the normal isozymes in electrophoretic. mobility. A single variant was detected in one case of untreated leukemia; a similar component and...

Much work, using both histochemical and standard biochemical techniques, has been carried out on the phosphatases of peripheral nerve. It is known that this tissue contains both alkaline (Landow, Kabat, and Newman, 1942) and acid phosphatases (Wolf, Kabat, and Newman, 1943), and the changes in the levels of these enzymes in nerves undergoing Wallerian degeneration following transection have bee...

Alkaline phosphatase of intestinal origin hydrolyzed the S-substituted monoesters of phosphorothioic acid of the type RSP03Na2 (R = -CH2CH2NH2, -CH,CH,NHCOCH,, -CH,COO, or -CH2CHsCOOCzHs) at the S-P bond to yield orthophosphate and the corresponding thioalcohols. The rate of enzymic hydrolysis of cysteamine S-phosphate was measured at pH 9.0 in 0.1 N sodium barbital buffer at different concentr...

Contributions from Cori’s laboratory by Colowick et al. (3, 4) and Sutherland (5) have proposed the concept that the effects of certain hormones (from the anterior pituitary, adrenal cortex, and pancreas) in carbohydrate metabolism are iinked with their action on specific tissue enzymes. The action of insulin as thus far uncovered by the above investigators is not simple, and involves in skelet...

Since the publication by Kay (9) of a method for the determination of the glycerophosphatase of blood serum, increasing interest has been manifested in the occurrence of phosphatases in the blood and in various organs in health and disease. It was early established that the phosphatase of bone has its maximum activity in alkaline solution (i7), and that that of the prostate gland is active only...

The present study was designed to identify alkaline phosphatases in non-permeabilized hyphal cells of the fungus Neurospora crassa by staining these enzymatic activities with a modified azo dye coupling method. Our strategy allowed the identification of three non-specific alkaline phosphatase activities, one of them possibly being a novel putative enzyme, which is not responsive to either Mg(2+...