The present study was aimed to evaluate the antimicrobial resistance and the presence of antibiotic resistance genes in Vibrios spp. isolated from seafood. A total of 72 isolates of Vibrio in 6 species including V. parahaemolyticus, V. vulnificus, V. alginolyticus, V. harveyi, V. mimicus and V. cholerae were examined. The results revealed that all isolates were expressing multiple antibiotic r...

The wild-type Streptomyces coelicolor ~3(2) harbours a plasmid, SCPr (Vivian, 197 I). This, and other strains bearing SCPI autonomously, have been called SCPI+. The plasmid is readily transmitted to SCPIderivatives in mixed culture. By interacting with the chromosome in various ways and at several alternative positions, SCPr is responsible for high frequencies of chromosomal marker transfer bet...

We have developed a new λ Red recombineering methodology for generating transient selection markers that can be used to transfer mutations between bacterial strains of both Escherichia coli and Salmonella enterica. The method is fast, simple and allows for the construction of strains with several mutations without any unwanted sequence changes (scar-free). The method uses λ Red recombineering t...

Resistance to different antibiotics was found in 26 of the 30 strains analyzed, more than 70% of the strains analyzed were resistant to carbenicillin and ampicillin and a significant correlation was found between the resistance to both antibiotics. Plasmids were found in 80% of the strains analyzed, and 11 different plasmid profiles were observed. The most common profile obtained had only a 21....

OBJECTIVES Since biofilm formation is the hallmark of Enterococcus faecalis isolates, the aim of this study was to quantify biofilm formation in the presence of subinhibitory concentrations of tigecycline. METHODS Interference of tigecycline on biofilm formation was spectrophotometrically quantified using 20 biofilm-producing E. faecalis isolates with tigecycline MICs of 0.12 (8 strains) or 0...

background :  pathogenic bacteria because of beta-lactam antibiotic resistance genes are dangerous to society. this resistance due to esbl genes, plasmids and transposons that by receiving or mutation occurs. the most important factor beta-lactam antibiotic resistance is beta lactamase enzymes. the purpose of this study was to evaluate the frequency of hospital opportunistic pathogenic bacteria...

The biological activity of Streptomyces aureofaciens MY18 and Streptomyces roseviolaceus MR13 (local isolates) as influenced by temperature,aeration, different media and carbon and nitrogen sources were investigated. It was found that biomass., growth parameters and antibiotic activity of bOth strains were highly affected by these factors. The first strain showed higher temperature range than t...

The antibacterial activity of sisomicin (SS), a new aminoglycoside antibiotic active toward clinical isolates of Pseudomonas aeruginosa, was determined and compared with that of the gentamicin C complex. Both drugs were effective against these strains and showed almost the same antibacterial activity. A few strains were found to be resistant to SS. The antibiotic was inactivated by a cell-free ...