The sip gene encoding for a conserved highly immunogenic surface protein of Streptococcus agalactiae was amplified using polymerase chain reaction (PCR) and subcloned into prokaryotic expression vector pET32a (+) and expressed as a recombinant protein in E. coli BL21 (DE3). An indirect enzyme linked immunosorbent assay (ELISA) was developed using the purified Sip protein as a coating antigen, w...

Platelets are anucleate blood cells, which play an important part in haemostasis and maintenance of the integrity of blood-vessel endothelium. Interaction of platelet glycoproteins (GPs) with extracellular matrix proteins is critical to this function. The original impetus for investigating the alloantigens found on the GPs of the platelet membrane came from clinical observations of pathological...

Organ transplantation is the most effective treatment for end stage organ failure. More than 500,000 patients have been transplanted with heart, lung, kidney, liver or small bowl allografts in the United States of America. There are more than 100,000 patients waiting for lifesaving organ transplants in the United States of America, mainly due to limited numbers of donors. It would be ideal if t...

This study describes the fabrication of an aflatoxin (AFB1) electrochemical immunosensor by immobilization anti-AFB1 antibody-bovine serum albumin (BSA) conjugate on a layer L-cysteine (L-Cys) used to link antibody palladium nanoparticle-boron nitride (PdNP-BN)-modified carbon felt (CF) electrode. Each step was characterized using cyclic voltammetry (CV) and impedance spectroscopy (EIS). These ...

Using an indirect immunofluorescence technique liver membrane antibodies of IgG and IgA class have been demonstrated in a statistically significant proportion of sera from patients with alcoholic hepatitis and alcoholic cirrhosis. IgG and IgA class antibodies were found respectively in 23 and 25% of 48 patients with alcoholic hepatitis, in 27 and 33% of 84 with active cirrhosis, and 67 and 58% ...

Using an indirect immunofluorescence technique liver membrane antibodies of IgG and IgA class have been demonstrated in a statistically significant proportion of sera from patients with alcoholic hepatitis and alcoholic cirrhosis. IgG and IgA class antibodies were found respectively in 23 and 25% of 48 patients with alcoholic hepatitis, in 27 and 33% of 84 with active cirrhosis, and 67 and 58% ...