Here, we report the complete genome sequence for an isolate of Pseudomonas stutzeri that is highly competent for natural transformation. This sequence enables insights into the genetic basis of natural transformation rate variations and provides an additional data point for genomic comparisons across a ubiquitous and highly diverse bacterial species.

We present an approximate analytic study of our previously introduced model of evolution including the effects of genetic exchange. This model is motivated by the process of bacterial transformation. We solve for the velocity, the rate of increase of fitness, as a function of the fixed population size, N. We find the velocity increases with ln N, eventually saturating at an N which depends on t...

A small microcosm, based on optimized in vitro transformation conditions, was used to study the ecological factors affecting the transformation of Acinetobacter calcoaceticus BD413 in soil. The transforming DNA used was A. calcoaceticus homologous chromosomal DNA with an inserted gene cassette containing a kanamycin resistance gene, nptII. The effects of soil type (silt loam or loamy sand), bac...

In 1928, Griffith [1] reported that soluble substances from virulent pneumococcal cells trans‐ formed non-virulent pneumococcus to virulent forms. This substance has now been demon‐ strated to be DNA [2-4]. This is considered to be the first report on genetic transformation of bacteria by exogenous DNA. Subsequently, natural competence of Bacillus subtilis was re‐ ported in 1958 by Young and Sp...

conclusions the bacterial isolates exhibited strong biofilm formation potential, while the extracts of acacia arabica significantly inhibited biofilm formation among the isolates and, therefore, could be executed in the development of cost-effective biofilm inhibitor medicines. results pseudomonas aeruginosa (kc417303-05), staphylococcus hemolyticus (kc417306), and staphylococcus hominis (kc417...

Ethylene evolution from plants inhibits Agrobacterium-mediated genetic transformation, but the mechanism is little understood. In this study, the possible role of ethylene in Agrobacterium-mediated genetic transformation was clarified. It was tested whether or not plant ethylene sensitivity affected genetic transformation; the sensitivity might regulate bacterial growth during co-cultivation an...

Natural genetic transformation is widely distributed in bacteria and generally occurs during a genetically programmed differentiated state called competence. This process promotes genome plasticity and adaptability in Gram-negative and Gram-positive bacteria. Transformation requires the binding and internalization of exogenous DNA, the mechanisms of which are unclear. Here, we report the discov...

Nitrate pollutants increase the growth of algal bloom, resulting in fresh water eutrophication. The high nitrogen level in wastewater has become a growing concern, which has risen the necessity to develop efficient nitrogen removal techniques. Biological denitrification, which is the reduction of oxidized nitrogen compounds like nitrate or nitrite to gaseous nitrogen compounds, is the most impo...

Nitrate pollutants increase the growth of algal bloom, resulting in fresh water eutrophication. The high nitrogen level in wastewater has become a growing concern, which has risen the necessity to develop efficient nitrogen removal techniques. Biological denitrification, which is the reduction of oxidized nitrogen compounds like nitrate or nitrite to gaseous nitrogen compounds, is the most impo...

Horizontal gene transfer (HGT) is recognized as the major force for bacterial genome evolution. Yet, numerous questions remain about the transferred genes, their function, quantity and frequency. The extent to which genetic transformation by exogenous DNA has occurred over evolutionary time was initially addressed by an in silico approach using the complete genome sequence of the Ralstonia sola...