Shoot tips of two fig cultivars (Conadria and Black Mission) proliferated in vitro, were cultured on modified MS medium supplemented with 0.5 mg/l 6-benzylaminopurine (BAP). The present investigation aimed to investigate the effect of different medium salt strengths (Double, full, half and quarter) and different sources of carbon (sucrose and fructose) at different concentrations (0.1 and 0.2 m...

In vitro propagation of Dendrobium macrostachyum, a threatened and endemic species was achieved through nodal explants. The nodal explants were cultured on Murashige and Skoog (MS) basal medium and MS medium supplemented with N6-benzyladenine (BA-2.22, 4.44 and 8.88 microM), Kinetin (KN-2.32, 4.65, and 9.29 microM) and Coconut water (CW, 5, 10 and 15%) individually or in combination with 2.69 m...

A simple and efficient plantlet regeneration protocol via direct organogenesis was established for camphor tree (Cinnamomum camphora L.). Stem segments with one node (SN explants) from embryo-cultured seedlings (EC seedlings) were used as explants. Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2, 4-dichlorophenoxyacetic acid and 2.0 mg/L 6-benzyladenine was used to induce cotyledon...

The uptake of glucose and of 3-O-methyl-d-glucose by cormel slices of Gladiolus X gandavensis Van Houtte was studied in relation to cormel dormancy. Uptake was higher in nondormant cormels. Incubation of nondormant cormels with abscisic acid (ABA) reduced their uptake capacity. Treatment of dormant cormels with 6-benzyladenine (BA) did not affect their uptake rate. ABA and BA promoted O(2) upta...

The relationship of 6-benzylaminopurine (BA)-induced fruitlet abscission to seed number and endogenous cytokinin levels in the fruit and leaves was examined in `McIntosh' apples (Malus domestica Borkh.). BA applied at the 10 mm stage of fruit development effectively thinned apple fruit and increased fruit size. The seed number of abscising fruit increased as BA concentrations increased. In pers...

By repeated selection of pigment portions of tissue the red callus induced from root seed­ lings of Penstemon serrulatus Menz. was chosen for suspension culture, which was maintained in Schenk and Hildebrandt medium supplemented with naphthaleneacetic acid (0.2 mg/1), 6-benzylaminopurine (2 mg/1) and sucrose (50 g/1). From the cultured cells eight phenolic compounds were isolated. They were ide...

Common bean (Phaseolus vulgaris L.) is an economic important crop and one of the major grain legumes for human consumption in Latin America, Africa and Asia. A morphological study of shoot induced from embryonic axes development in four Costa Rican bean varieties (Brunca, Huetar, Guaymi and Bribri) cultivated on MS media with or without 5 mg·L de N-benzylaminopurine (BAP) by scanning electron m...

The present study was undertaken to investigate the possibility for developing Efficient Plant regeneration protocol in Vigno mungo L. For this, the effect of Cytokinens on in vitro regeneration from Cotyledonary explants was studied in Black gram (Vigno mungo L.) Hepper Var Vamban-1. The cytokinins used were 6-benzylaminopurine (BAP), Thidiazuron (TDZ), and kinetin (Kn). The regenerated shoots...