Opening of the permeability transition pore (PTP) has been implicated as an important mitochondrial event that occurs during apoptosis. We examined the role of the PTP in the well-characterized cell death of rat sympathetic neurons deprived of nerve growth factor (NGF) in vitro. Removal of NGF causes these neurons to undergo either a classic apoptotic cell death or, when treated with a broad-sp...

Barrier to autointegration factor (BAF) is a DNA-binding protein found in the nucleus and cytoplasm of eukaryotic cells that functions to establish nuclear architecture during mitosis. Herein, we demonstrate a cytoplasmic role for BAF in host defense during poxviral infections. Vaccinia is the prototypic poxvirus, a family of DNA viruses that replicate exclusively in the cytoplasm of infected c...

The uptake and bioaccumulation of 15 road dust metals by the zebra mussel (Dreissena polymorpha) were investigated in laboratory exposure studies with emphasis on the traffic-related platinum-group elements (PGEs) palladium (Pd), platinum (Pt), and rhodium (Rh). The biological availability of the metals may depend on water characteristics, so the mussels were maintained in two types of water: n...

east of China. It has long been used as a traditional crude drug for the treatment of bronchial asthma and chronic bronchitis. Previous investigations of the whole plants of E. lanulata have yielded kaempferol, quercetin, kaempferol 3-O-Lrhamnoside, quercetin 3-O-L-rhamnoside, 6,7-dihydroxycoumarin and maoyancaosu. More than 30 types of cytokines and growth factors have been identified as ligan...

The present study aimed to investigate the impact of sewage sludge (SS) amendment on growth, yield, and biochemical attributes marigold (Tagetes erecta L. var. Pusa Basanti Gainda) crop. For this purpose, flowers were cultivated using three different treatments SS, i.e., 0% (control with no SS), 5%, 10%. Multiple linear regression (MLR) modeling was performed develop prediction models for soil ...

Eukaryotic cells store their chromosomes in a single nucleus. This is important to maintain genomic integrity, as chromosomes packaged into separate nuclei (micronuclei) are prone to massive DNA damage. During mitosis, higher eukaryotes disassemble their nucleus and release individualized chromosomes for segregation. How numerous chromosomes subsequently reform a single nucleus has remained unc...

Assembly of the nuclear envelope (NE) in telophase is essential for higher eukaryotic cells to re-establish a functional nucleus. Time-lapse, FRAP and FRET analyses in human cells showed that barrier-to-autointegration factor (BAF), a DNA-binding protein, assembled first at the distinct ;core' region of the telophase chromosome and formed an immobile complex by directly binding with other core-...

Three centipede species (Clinopodes flavidus, Cryptops anomalans and Eupolybothrus transsylvanicus) were used as bioindicators of trace metal metalloid pollution in Belgrade, Serbia. The concentrations 13 elements (the metals Mn, Co, Ni, Cu, Zn, Rb, Sr, Cd, Tl, Pb U metalloids As, Se) whole animals soil measured by inductively coupled plasma mass spectrometry (ICP-MS). Differences the some anal...

Nuclear lamin filaments and associated proteins form a nucleoskeletal ("lamina") network required for transcription, replication, chromatin organization and epigenetic regulation in metazoans. Lamina defects cause human disease ("laminopathies") and are linked to aging. Barrier-to-autointegration factor (BAF) is a mobile and essential component of the nuclear lamina that binds directly to histo...