Blood serum is incubated with ascorbic acid and hydrochloric acid to liberate protein-bound iron. Proteinsare precipitated with trichloroacetic acid in the presence of chloroform. To an aliquot of clear supernatant is added a solution of tripyridyltriazine and sufficient ammonium acetate to adjust the pH between 4 and 5. Absorbance of the blue-colored complex is measured at 590 m. Iron-binding ...

Our previous research has demonstrated that dissolved organic matter (DOM) influences calcium carbonate mineral formation in surface and ground water. To better understand DOM mediation of carbonate precipitation and DOM co-precipitation and/or incorporation with carbonate minerals, we characterized the content and speciation of DOM in carbonate minerals and in the lake water of Pyramid Lake, N...

Opa1, also known as Mgm1 in yeast, is a mitochondrial member of the dynamin family. Unlike other dynamin family members, Opa1 has an N-terminal mitochondrial targeting sequence, suggesting that this protein is imported into mitochondria. Here, we describe biochemical techniques, such as mitochondrial isolation, digitonin extraction, a protease protection assay, and carbonate extraction, that we...

9 We report oxygen and carbon isotope results of bulk and detrital carbonate grains 10 from the last glaciation at IODP Site U1308 (re-occupation of DSDP Site 609). Oxygen 11 isotopic values of individual detrital carbonate grains from Heinrich events average 12 -5.4‰±1.3‰, presumably reflecting values of limestone and dolomite derived from 13 lower Paleozoic basins in northern Canada and north...

A simplified technic for the determination of serum protein-bound iodine (PBI) is presented. Endogenouslylabeled 1131 PBI is completely recovered. The technic is basedon the method of Barker et al. (1) and includes the following modifications: The number of washesof the precipitated serum proteins are decreased. Unashed iodide standards are used in a sodium carbonate solution rather than an int...

Conversion of glycerol from biodiesel production to glycerol carbonate was studied by esterification with dimethyl carbonate in a non-catalytic supercritical condition. It was found that in a non-catalytic supercritical condition, glycerol at higher purity gave higher yield of glycerol carbonate at 98 wt% after reaction at 300 °C/20-40 MPa/15 min. The yield of glycerol carbonate was observed to...

BACKGROUND Sevelamer carbonate is an improved, buffered form of sevelamer hydrochloride developed for the treatment of hyperphosphataemia in CKD patients. Sevelamer carbonate formulated as a powder for oral suspension presents a novel, patient-friendly alternative to tablet phosphate binders. This study compared the safety and efficacy of sevelamer carbonate powder with sevelamer hydrochloride ...

Authigenic carbonate was recently invoked as a third major global carbon sink in addition to primary marine carbonate and organic carbon. Distinguishing the two carbonate sinks is fundamental to our understanding of Earth's carbon cycle and its role in regulating the evolution of atmospheric oxygen. Here, using microscale geochemical measurements of carbonates in Early Triassic strata, we show ...