Colchicine has been used to induce chromosome doubling in plant species. Diploid cotton species have inherited desirable genes that are resistant to biotic and abiotic stresses and also possesses useful fiber quality traits, such as higher fiber length and strength. To transfer these desirable traits to the most extensively cultivated tetraploid cotton species through hybridization, it is a pre...

BRO human melanoma cells, obtained from a biopsy of a highly aggressive and malignant primary tumor, were grown as xenografts in nude mice and in cell culture. These cells were exceptionally tumorigenic and malignant for nude mice. NIH-II nude mice survived 11.0 +/- 0.4 (S.E.) and 14.1 +/- 0.4 days after i.p. inoculation of 10(7) or 10(6) BRO cells, respectively, and lethal tumors developed in ...

Doubled haploid technology has become an increasingly popular and important tool for developing new maize lines (Zea mays L.) breeding. Conventional breeding requires repeated self-pollination about six to ten generations obtain homozygous inbred lines. Completely can be produced in only two by using doubled technology. In vivo induction of the maternal method was used production 32 different d...

We announce the release of chromEvol version 2.0, a software tool for inferring the pattern of chromosome number change along a phylogeny. The software facilitates the inference of the expected number of polyploidy and dysploidy transitions along each branch of a phylogeny and estimates ancestral chromosome numbers at internal nodes. The new version features a novel extension of the model accou...

We announce the release of chromEvol version 2.0, a software tool for inferring the pattern of chromosome number change along a phylogeny. The software facilitates the inference of the expected number of polyploidy and dysploidy transitions along each branch of a phylogeny and estimates ancestral chromosome numbers at internal nodes. The new version features a novel extension of the model accou...

A molecular, anatomical and cytogenetic study of an interspecific hybrid between Manihot esculenta (cassava) and the wild species M. oligantha was carried out. Cytogenetics revealed relatively complete chromosome pairing and high viability of the pollen grains. Ovule structure examined by the clearing method showed polyembryony in 2.7% of the ovules. Doubling of the chromosome number resulted i...

This study was aimed to obtain polyploid plants of viola (Viola acuminata Ledebour) using the antimitotic agent colchicine. Experiments consisted in using colchicine on ungerminated seeds. The seeds were plunged into colchicine solutions in concentrations of 0.01, 0.05, 0.10 and 0.20% for 24 and 48 h. Polyploidy levels (diploid, tetraploid and hexaploid) were firstly detected by chromo...

Aegilops tauschii (2n=2x=14), the D-genome donor is closest progenitor to modern wheat representing a large proportion of unexplored genetic variation. Flourishing in adverse climatic conditions it can be utilized improvement programmes. We targeted develop and characterize primary synthetics amphiploids from nine different accessions Ae. one Triticum durum cv. PBW114 through spontaneous chromo...

IX root development, cell divisions at the root tip are predominantly localized in the first few millimeters of the typical dicot seedling root. These divisions give rise to the cells which form the mature root structure. By differential enlargement and maturation processes these cells form the distinctive mature tissue systems of the primary root: the epidermis, cortex, endodermis, pericycle, ...