The emergence of multidrug-resistant tuberculosis calls for new, rapid drug susceptibility tests. We have tested 150 Mycobacterium tuberculosis isolates against the second-line drugs ethionamide, kanamycin, capreomycin, ofloxacin, and para-aminosalicylic acid by the colorimetric resazurin microtiter assay and the proportion method. By visual reading, MICs were obtained after 8 days. A very good...

"Naked eye" color detection of proteins was achieved by embedding calixarene receptors within vesicles comprising phospholipids and the chromatic polymer polydiacetylene. Dramatic visible absorbance changes were induced through electrostatic interactions between the protein surface and the vesicle-incorporated hosts. The colorimetric responses could be induced by micromolar protein concentratio...

The development of new sensitive, cost-effective and user-friendly colorimetric bioassays is in increasing demand to meet the requirement of modern clinical diagnostics and field detection. Herein, a novel iron oxide-to-Prussian blue (PB) nanoparticle (NP) conversion strategy was developed and applied to sensitive colorimetric immunosensing of cancer biomarkers. In a typical sandwich-type immun...

We have evaluated three analytical methods for determining inorganic phosphorus in serum applied to the Technicon RA-I000 analyzer: a fully enzymatic colorimetric method based on the specific system purine nucleoside phosphorylase/xanthine oxidase coupled to an indicator colorimetric reaction similar to the Trinder reaction; a chemical method involving the direct UV measurement of the phosphomo...

The RGB signals generated by different cameras are not equal for the same scene. Therefore, cameras are characterized based on a CIE standard colorimetric observer. One method of deriving a colorimetric characterization matrix between camera RGB output signals and CIE XYZ tristimulus values is least squares polynomial modeling. Yet, this involves tedious experiments to obtain a camera transfer ...

Spectrophotometric measurement applied on saturated granular activated carbon (GAC) is not yet explored. A colorimetric method in the visible range has been developed in order to determine the exhaustion level of GAC used in rum production. Aqueous ammonia solution has been used as an indicative agent to determine the extraction rate of taste compounds within the rum production process and the ...

An algorithm is proposed for the spectral and colorimetric characterization of digital still cameras (DSC) which allows them to be used as tele-colorimeters with CIE–XYZ color output, in cd/m2. The spectral characterization consists in the calculation of the color-matching functions from the previously measured spectral sensitivities. The colorimetric characterization consists in transforming t...

The present paper studies two different table-based approaches for the calibration of electronic imaging systems. The first approach, which is the classical one,uses the device-independent CIE-XYZ colorimetric space as an intermediate standard space. Input and output devices such as scanners, displays and printers are calibrated separately with respect to the objective CIE-XYZ space. The calibr...

We describe two fully enzymic methods, fluorometric and colorimetric, for determination of triglycerides (triacylglycerols) in serum. Samples are incubated with microbial lipase for 10 min, and the glycerol released from the triglycerides is oxidized by NAD+ in the presence of glycerol dehydrogenase. In the fluorometric method, the resulting NADH is in turn oxidized by resazurin as catalyzed by...