AIM The aim was to assess the seroprevalence of B. abortus and Leptospira hardjo in the cattle population of Bihar, this work was carried out. MATERIALS AND METHODS Randomly selected 450 cattle from nine districts of Bihar were serologically screened for antibodies against L. hardjo and B. abortus. DAS-ELISA for leptospira and AB-ELISA for brucella were carried out. Based on the results preva...

Two methods (DAS-ELISA and RT-PCR) were compared for the detection of Prunus necrotic ringspot virus (PNRSV) in woody host Prunus mahleb isolated from Malatya. Total RNA extractions were made from serially diluted fresh leaf tissue, root, bark and one year old green bark tissue using silica-based method. Purified total RNA extracts were used as template for cDNA synthesis by reverse transcripti...

Comparative tests using inmunosorbent electron microscopy plus decoration and ELISA with antibodies against Garlic virus A (GarV-A) and Garlic virus C (GarV-C) coat proteins showed that they specifically recognize the respective viruses. On this basis, the GarV-A antiserum was used in DAS-ELISA tests to study the distribution of GarV-A in different regions of Argentina. A total of 2655 samples,...

abstract the pvyntn strain induces potato tuber necrotic ringspot disease ptnrd . pvyntn strain is sub group of tobacco necrosis veinal pvyn strain . pvyntn strain is a recombinant between pvyn and pvyo strains . symptoms are often totally symptomless or undetectable by visual inspections at harvest , usually are apparent during post harvest in storage conditions and induce to decrease quality ...