Objective: Increased risk of atherosclerotic cardiovascular disease in subjects with type 2 diabetes is linked to elevated levels triglyceride-rich lipoproteins and their remnants. The metabolic effects PCSK9 (proprotein convertase subtilisin/kexin 9) inhibitors on this dyslipidemia were investigated using stable-isotope-labeled tracers. Approach Results: Triglyceride transport the metabolism a...

The acute reduction of low-density lipoprotein (LDL) cholesterol obtained by LDL-apheresis allows the role of the high level of circulating LDL on lipoprotein metabolism in heterozygous familial hypercholesterolemia (heterozygous FH) to be addressed. We studied apolipoprotein B (apoB) kinetics in five heterozygous FH patients before and the day after an apheresis treatment using endogenous labe...

Five different mouse monoclonal antibodies were raised against apoprotein B, the major protein in human low-density lipoproteins (LDL). Competitive binding studies suggested that these antibodies recognized four different epitopes on the lipoprotein particle. All antibodies bound to very-low-density and intermediate-density lipoproteins, which are the metabolic precursors of LDL and also contai...

Serum Triglyceride and cholesterol were estimated by enzymatic method and routine method (Wybanga method) (10). HDL was determined by phosphotunstate/magnesium method fo l lowed by Wybanga method. (Serum is f ree f rom LDL, VLDL and Chlomicron). Low–density lipoprotein (LDL) and very low–density lipoprotein (VLDL) were found out by formula utilizing the values of cholesterol, Triglycerides, HDL...

BACKGROUND There are limited data regarding qualitative lipoprotein abnormalities in undialysed uremic patients without proteinuria. In this report, we focused on lipoprotein changes observed in uremic patients with proteinuria as well as on the susceptibility of low-density lipoprotein (LDL) of these patients to oxidative modification in vitro. METHODS 20 patients with chronic renal failure ...

OBJECTIVE Cholesteryl ester transfer protein (CETP) inhibition with torcetrapib not only increases high-density lipoprotein cholesterol levels but also significantly reduces plasma triglyceride, low-density lipoprotein (LDL) cholesterol, and apolipoprotein B (apoB) levels. The goal of the present study was to define the kinetic mechanism(s) by which CETP inhibition reduces levels of apoB-contai...

In this study, the effect of lovastatin and fluvastatin was compared with placebo in patients with high levels of total cholesterol and low density lipoprotein cholesterol (LDL-C) on the plasma lipid profile. In a prospective single blind clinical trial with convenient sampling, 120 hypercholesterolemic men and women with Tcholesterol ≥220 mg/dL, LDL-C ≥ 160 mg/dL, and triglyceride ≤ 350 mg...

Tumor gangliosides are biologically active (immunosuppressive and tumor-enhancing) cell surface molecules which are shed into the circulation in vivo. However, the mechanism of transport of these molecules (i.e., in solution or bound to proteins or other lipids) is not known. To resolve this question we traced, by direct chemical detection, the serum localization of a specific human tumor gangl...

The relationship between the concentrations of intermediate-density lipoprotein (IDL) and other lipoproteins and the extent of coronary artery disease (CAD) was studied in 182 consecutive patients evaluated by selective coronary cineangiography. On univariate analysis, the extent of CAD correlated significantly and positively with very low density lipoprotein (VLDL) cholesterol, IDL cholesterol...