Respiratory virus infections are the main cause of infant hospitalization and are potentially severe in children with congenital heart disease (CHD). Rapid and sensitive diagnosis is very important to early introduction of antiviral treatment and implementation of precautions to control transmission, reducing the risk of nosocomial infections. In the present study we compare different technique...

Giardia lamblia cysts in fecal specimens are detected by conventional morphological methods. The direct immunofluorescence monoclonal antibody assay (DFA) is also applied to detect G. lamblia cysts in feces, but little is known about the usefulness of DFA in fecal specimens stored under various conditions. The aim of the present study was to evaluate the DFA for detection of G. lamblia cysts an...

objective: confocal laser scanning microscopy (clsm) is relatively a new light microscopical imaging technique with a wide range of applications in biological sciences. primary value of clsm for the biologist is its ability to provide optical sections from three-dimensional specimen. the present study was designed to assess the thickness content of in vivo accumulated dental plaque using clsm a...

conclusions the rabies virus np could be extracted and purified in an appropriate amount from infected cell culture. the results of sds-page analysis showed that the intact rabies virus np had been purified properly and thus could be used for further steps to produce the specific diagnostic rabies conjugate. results the volume of the lysate was 15 ml and it became 2.5 ml after purification, wit...

The continuous development of the vast arsenal of fabrication techniques is a pivotal factor in the breakthrough of nanotechnology. Although the broad interest is generally focused on the reduction of the dimensions of the fabricated structures, localized functionalization of the nanomaterials emerges as a key factor closely linked to their potential applications. In particular, fabrication of ...

Fluorescent antibody methods were originally introduced by Coons and associates in 1942. Included in this first publication was a procedure for antibody-fluorescein conjugation and a description of fluorescent reactions obtaincd by means of ultraviolet light microscopy. This communication, and later ones by other authors, indicated that fluorescent antibody procedures were practical for rapid a...