BACKGROUND Therapeutic angiogenesis with vascular endothelial growth factor (VEGF), delivered either via recombinant protein infusion or via gene therapy, has shown promise in preclinical models of various diseases including myocardial infarction, renovascular disease, preeclampsia, and neurodegenerative disorders. However, dosing, duration of expression, and tissue specificity are challenges t...

Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to recover the ELPylated antigens and antibodies from plant material. The functionality of both the ...

Let G(V; E) be a graph, and let f : G ! R 2 be a one to one function that produces a layout of a graph G on the plane. We consider the problem of assigning text labels to every edge of the graph such that the quality of the labeling assignment is optimal. This problem has been rst encountered in automated cartography and has been referred to as the Line Feature Label Placement (LFLP) problem. E...

OBJECTIVES Hepatitis B virus (HBV) is a member of the hepadnavirus family. The HBV genome contains four genes designated as S, C, P, and X. The HBV X (HBx) gene encodes for a 16.5-kDa regulatory protein that enhances HBV replication and exerts multifunctional activities. The aim of this study is to describe the rapid and easy purification of HBx using ELP (elastin-like polypeptide) fusion prote...

Elastin-like polypeptides (ELPs) are environmentally responsive biopolymers based on the elastinderived pentapeptide repeat Val-Pro-Gly-Val-Gly. ELPs undergo a reversible phase transition termed an “inverse temperature transition” (Urry 1992, 1997). Below their transition temperature (Tt), the polypeptides are highly soluble in aqueous solutions. However, when the temperature is raised above Tt...

Recent observations in extreme-ultraviolet (EUV) wavelengths reveal a new late phase some solar flares, which is seen as second peak warm coronal emissions ($\sim3$ MK) several tens of minutes to few hours after the soft X-ray (SXR) peak. The origin EUV (ELP) explained by either long-lasting cooling process long ELP loops, or delayed energy ejection into loops well main flare heating. Using wit...

It has been hypothesized that invasive plant species with extended leaf phenology (ELP) elevate generalist herbivore populations, increasing herbivory on native plants (apparent competition). This hypothesis assumes that consumption of the invasive is associated with periods of ELP, the invasive is a major component of the herbivore's diet, and that it is more nutritious than native plants duri...

BACKGROUND Plant bioreactor offers an efficient and economical system for large-scale production of recombinant proteins. However, high cost and difficulty in scaling-up of downstream purification of the target protein, particularly the common involvement of affinity chromatography and protease in the purification process, has hampered its industrial scale application, therefore a cost-effectiv...

background and objective: use of elastin-like proteins (elps) provides high-performance protein purification without need for chromatography. in line with cost reduction and facilitation of recombinant proteins purification, which represent a high percentage of production costs, in this project, we eliminated the need for proteases in the process of separation of recombinant proteins from elp b...