the effect of in planta tiba and proline on in vitro seedlings and cell culture of sugar beet was investigated. sterilized seeds were grown in vitro on ½ strength ms medium supplemented with 0 or 3 mg/l tiba. calli obtained on young leaves cultured on ms medium containing 1 mg/l bap, were used for the intiation of cell suspension cultures using ms basal composition supplemented with 0 or 50 m m...

Background Mesenchyme stem cells (MSCs) are one of the widely used sources in tissue engineering and efficacy of cultural environment. In this survey, rat stem cells are used as coculture. Owing to main obstacle of embryo culture in the lab medium, the survival of embryo in lab is low in comparison with that of normal conditions. The purpose of this research is the survey of fertilization and c...

The aim of this study was to identify the in vitro development stage at which the culture of a single or low number (n = 5 or 10) of oocytes/embryos could impair development in comparison with culture in group (n = 50). In the Experiment 1, it was confirmed that single in vitro embryo production yielded lower cleavage and blastocyst rates than in group (49.4 vs. 83.0%; 0% vs. 37.8%, respectivel...

This study involves in vitro androgenesis of Zea mays L. via anther culture. In the first experiment, three embryo induction media were tested in ETH-M82 genotype of maize. The results showed that the embryo structures were established 2 to 3 weeks after culturing the anthers. The effect of induction medium was signifi­cant for embryo frequencies. The obtained results confirm that the induction...

STUDY QUESTION Is the amount of cell-free DNA released by human embryos into culture medium correlated with embryo morphological features? SUMMARY ANSWER The mitochondrial DNA (mtDNA) content of culture medium is significantly associated with the fragmentation rate on Days 2 and 3 of embryo development, whether the oocyte came from women ≤ 35 or >35 years old. WHAT IS KNOWN ALREADY Cellular...

Background: In vitro culture of ovarian follicles may preserve fertility in women with premature ovarian failure due to cancer .It seems that creation a condition that could maintain cellular communications and supports growth of follicles to produce mature oocytes appear to be essential. Nitric oxide (NO) has been recently shown to act with a dual action in mouse oocyte meiotic maturation depe...

Recent studies have suggested that leptin plays an important role in reproduction. Ob-R is expressed in the murine embryo, and is suggested to play a role in embryo development, although contradictory results have been reported. In the present study, Ob-R expression was observed both at the mRNA and protein levels in porcine early embryos. We have also demonstrated that leptin is produced in th...

A key factor in the use of assisted reproductive technologies (ART) for diverse species is the safety of procedures for long-term health. By using a mouse model, we have investigated the effect of in vitro culture and embryo transfer (ET) of superovulated embryos on postnatal growth and physiological activity compared with that of embryos developing in vivo. Embryo culture from two-cell to blas...

embryo culture were used to produce inter specific walnut hybrid plants between juglans nigra l. and j. regia l. developed ovaries were collected from j. rejia female flowers which were pollinated with j. nigra pollen grain by high dusting on a mature tree. in order to isolate embryos, exocarps were aseptically removed. isolated embryos from ovules at different developmental stages were transfe...

Conceptuses from 9.5-day pregnant rats were cultured for 48 h in heat-inactivated homologous serum to which leupeptin, a specific inhibitor of the lysosomal cysteine-proteinases, was added for the final or the penultimate 6 h. The presence of leupeptin (25 micrograms/ml or above) increased the protein content of yolk sacs at harvesting to approximately twice the control value. The protein conte...