L-Asparaginase has remarkable properties which make it useful in dual pharmaceutical and food industries.In this study, simple and advantageous method has been validated for rapid and precise determination of intracellular L-Asparaginasein bacterial species. A suspension of bacterial cells was used instead ofcell extract and incubated by substrate (asparagine) after simple wash and centrifugati...

The concentration of plasma sialic acid was estimated using the modified chemical method and the more sensitive enzymatic method in 20 subjects with impaired glucose tolerance and 20 control subjects. The mean sialic acid concentration values of the control subjects and subjects with impaired glucose tolerance using the enzymatic method were 1.747 +/- 0.047 and 2.583 +/- 0.070 mmole/l and 1.753...

11 HASSID and ABRAHAM, Chemical Procedures for Analysis of Polysaccharides, in: Methods of Enzymology, Vol . 3, Academic Press, New York 1957. 12 F. W. FALES, J. biol. Chemistry 193, 113 [1951] . 13 N. NELSON and M. SOMOGYI, as cited in Practical clinical B i o c h e m i s t r y , H . V A R L E Y , 4 t h e d i t i o n , W . HEINEMANN, Medical Book Ltd., London 1967. 14 O. FOLIN and H. Wu, as ci...

This review describes the life of Sir John Cornforth AC CBE FRS, who was awarded the Nobel Prize for Chemistry in 1975. It covers his early life in Australia, his work in Oxford, the National Institute for Medical Research, the Milstead Laboratory of Chemical Enzymology and the University of Sussex, together with the contributions made by his wife, Lady Rita Cornforth.

background: the clinical diagnosis of meningitis is crucial, particularly in children. the early diagnosis and empiric an­tibi­otic treatments have led to a reduction in morbidity and mortality rates. pcr and the enzymatic digestion of 16srdna frag­ment which is produced by universal primers led up fast and sensitive determination. the purpose of this study was to investi­gate a rapid method fo...

DNA glycosylases safeguard the genome by locating and excising a diverse array of aberrant nucleobases created from oxidation, alkylation, and deamination of DNA. Since the discovery 28years ago that these enzymes employ a base flipping mechanism to trap their substrates, six different protein architectures have been identified to perform the same basic task. Work over the past several years ha...

In enzymology, as in organic chemistry, many transformations revolve around the chemistry of the carbonyl group. A carbonyl group is electrophilic how can it be made more so? Hydrogens next to a carbonyl group have a reduced pK, how can this reactivity be promoted? Nature has found several ways to address these mechanistic challenges, the most important of which is the use of imines. Studies on...