BACKGROUND Colletotrichum truncatum is the most common pathogenic fungus associated with soybean anthracnose, a prevalent disease in Argentina. Pectinolytic enzymes are involved in the pathogenicity of a wide range of plant pathogenic fungi. OBJECTIVES To explore pectinolytic enzyme production in Argentinian Colletotrichum strains isolated from diseased soybean plants from different geographi...

berangan banana were treated with hot water at 50 ºc for 0 (control), 10, and 20 min and then kept at room temperature (25±2 ºc), 75-80% rh to observe the ripening progress. firmness and activity of cell wall degradation enzymes such as polygalacturonase (pg), pectin methylesterase (pme), and pectate lyase (pl) were determined for treated and untreated banana. the microstructure of banana peel ...

Submerged culture conditions for polygalacturonase production by Sclerotinia sclerotiorum (MR5) were optimized by Taguchi orthogonal array experimental design methodology. This approach facilitates the study of interaction of a large number of variables spanned by factors and their settings with a small number of experiments leading to considerable saving in time and cost for the process optimi...

Apple is a fruit with numerous nutrient content alongised high levels of harvest and consumption. The Manalagi one the varieties grown in Indonesia,characterized by yellowish green coloration, sweet taste, as well easy rot damage potentials. Hence, many people have encouraged need to process raw samples into processed juice products. This practice raises sale value prolongs shelflife. However, ...

Polygalacturonase inhibiting proteins (PGIPs) are major defensive proteins produced by plant cell walls that play a crucial role in pathogen resistance by reducing polygalacturonase (PG) activity. In the present study, a novel PGIP gene was isolated from tobacco (Nicotiana tabacum), hereafter referred as NtPGIP. A full-length NtPGIP cDNA of 1,412 bp with a 186 bp 5'-untranslated region (UTR), a...

Polygalacturonase [PG; poly(1,4-alpha-D-galacturonide) glycanhydrolase; EC 3.2.1.15] is expressed in tomato only during the ripening stage of fruit development. PG becomes abundant during ripening and has a major role in cell wall degradation and fruit softening. Tomato plants were transformed to produce antisense RNA from a gene construct containing the cauliflower mosaic virus 35S promoter an...

The developmental changes that accompany tomato fruit ripening include increased solubilization and depolymerization of pectins due to the action of polygalacturonase (PG). Two PG isoenzymes can be extracted from ripe fruit: PG2, which is a single catalytic PG polypeptide, and PG1, which is composed of PG2 tightly associated with a second noncatalytic protein, the beta subunit. Previous studies...

Previously identified fungal pectinase producers of the species Aspergillus sojae were used for optimization of polygalacturonase production in solid-state fermentation applying Design of Experiment. The effects of media composition and several process parameters, like inoculum size, moisture level, incubation time and temperature on polygalacturonase activity were studied in screening and opti...