Novel carbon fiber enzyme electrodes were constructed and evaluated. Fabrication of was performed via electrochemical deposition a conducting matrice composed polythiophene polypyrrole (PTh–PPy) onto substrates. The entrapped into the matrix during electropolymerization. Resultant biosensors represented higher kinetic parameters, Vmax Km, in comparison to PPy matrice, which are 2.471 ± 0.150 m...

Chemical tests for measurement of blood alcohols are the subject of much interest. particularly in legal cases, and there is a need for quick, simple and reliable assay methods. Likewise. the determinations of carboxylic acids and aldehydes are of importance in various industrial fields, but such assays are difficult to carry out. especially at low concentrations. In the past. alcohols_ have be...

In order to detect and quantify the phenolic contents of a wastewater with biosensors, two working electrodes based on modified Poly(Pyrrole) films were fabricated. Enzyme horseradish peroxidase was used as biomolecule of the prepared electrodes. Various phenolics were tested at the biosensor. Phenol detection was realized by electrochemical reduction of quinones produced by enzymatic activity....

A disposable amperometric biosensor for commercial use to detect hydrogen peroxide has been developed. The sensor is based on screen-printed carbon paste electrodes modified by electropolymerization of pyrrole with horseradish peroxidase (HRP) entrapped. The facture techniques of fabricating the enzyme electrodes are suitable for mass production and quality control. The biosensor shows a linear...

A detailed kinetic analysis of the pertinent physical processes underlying the operation of enzyme electrodes immobilized within alkane thiol self assembled monolayers is developed. These electrodes utilize a soluble mediator, which partitions into the monolayer, regenerates the active catalytic form of the enzyme and is re-oxidized at the underlying support electrode surface giving rise to a c...

This study aims to characterize the phosphate buffer and urease enzymes through UV-Vis SEM-EDS absorbance spectra using tungsten as an indicator electrode. The method used is a potentiometric biosensor with enzyme immobilization technique for urea analyte. A small detection range of 10-5-10-4M has been studied PVA-enzyme coated electrodes PVC-KTpClPB. On this basis, researchers increased by ana...