Abstract The regulation of the activity and selectivity phospholipase A2 (PLA2), which is capable cleaving fatty acid in second position ( sn-2 ) phospholipid, carried out through membrane-binding catalytic sites enzyme. For hydrolytic activity, PLA2 must first bind to phospholipid membrane, binding efficiency depends on composition membrane. site formed by several tens amino acids its differs ...

Copper is one of the heavy metal in plant that causes toxicity at high concentration via producing reactive oxygen species. Nitric oxide can protect cells from oxidative stress produce by reactive oxygen species. Effect of different concentrations of copper (1000, 1500 and 2000 μM) and interaction with nitric oxide (100 and 150 μM) were studied on growth parameters (shoot and root length) and s...

In order to investigate the effect of different levels of rapeseed meal on performance and content of triiodothyronine (T3), thyroxine (T4) and thyroid stimulating hormone (TSH) in broiler chickens’ blood, 640 1-d-old Ross male broiler chickens were studied through a 4 × 2 factorial arrangement including 4 levels of rapeseed meal (RSM) (0, 5, 10 and 15 percent) and two levels of enzyme (0 and 0...

ABSTRACT: Quantitative structure-activity relationship (QSAR) study on the piperidone-grafted mono- and bis-spirooxindole-hexahydropyrrolizines as potent butyrylcholinestrase (BuChE) inhibitors were carried out using statistical methods, molecular dynamics and molecular docking simulation. QSAR methodologies, including classification and regression tree (CART), multiple linear regression (MLR),...

Glyceraldehyde-3-phosphate dehydrogenase is a glycolytic enzyme that catalyses conversion of glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate. ATP has been found to have an inhibitory effect on this enzyme. To establish the interaction between the enzyme and ATP, a fluorescence technique was used. Fluorescence quenching in the presence of ATP suggests cooperative binding of ATP to the enzym...

Previous work indicates that aminoacyl-tRNA synthetases make a transient covalent adduct with cognate tRNAs, through Michael addition of an enzyme nucleophile to the carbon-6 position of uridine 8. We report the selective reduction of the 5,6 double bond of 4-thiouridine at position 8 in Escherichia coli tyrosine tRNA, so as to prevent formation of the presumed covalent enzyme-nucleic acid addu...

The binding of Cibacron blue F3GA to dihydrofolate reductase (EC 1.5.1.3) from chicken liver an amethopterin-resistant Lactobacillus casei has been studied by difference spectroscopy. The blue dye binds to the enzyme from each species in a specific fashion with a 1:1 stoichiometry. However, the mode of interaction of the dye with the enzyme and the site of interaction on the enzyme are very dif...

RecBCD enzyme is a heterotrimeric helicase/nuclease that initiates homologous recombination at double-stranded DNA breaks. Several of its activities are regulated by the DNA sequence chi (5'-GCTGGTGG-3'), which is recognized in cis by the translocating enzyme. When RecBCD enzyme encounters chi, the intensity and polarity of its nuclease activity are changed, and the enzyme gains the ability to ...